骨髓增生异常综合征和再生障碍性贫血患者骨髓原始细胞免疫表型分析

山东大学学报(医学版)

骨髓增生异常综合征和再生障碍性贫血患者
骨髓原始细胞免疫表型分析

赵广玲¹，许洪志¹，黄敏¹,李元堂²，武焕玲²，李建峰³，马春燕³

（山东大学附属省立医院 1. 血液科；2. 检验科；3. 中心实验室, 济南 250021）

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-02-16 发布日期:2009-02-16
通讯作者: 许洪志

Immunophenotyping in myelodysplastic syndrome and aplastic anemia

ZHAO Guangling¹, XU Hongzhi¹, HUANG Min¹, LI Yuantang²,
WU Huanling², LI Jianfeng³, MA Chunyan³

(1. Department of Hematology; 2. Department of Ecsomatics; 3. Central Laboratory,
Provincial Hospital Affiliated to Shandong University, Jinan 250021, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-02-16 Published:2009-02-16
Contact: XU Hongzhi

摘要/Abstract

摘要： 目的检测骨髓增生异常综合征（MDS）和再生障碍性贫血（AA）患者骨髓原始细胞免疫表型特征，探讨其对二者发病机制、诊断、分型诊断及鉴别诊断的临床意义。方法选用多种单克隆抗体，应用直接免疫荧光法、流式细胞术和CD45/SSC设门，对MDS组23例、AA组14例、正常对照组9例的骨髓原始细胞各免疫标志的表达率进行检测。结果MDS组与正常对照组相比其造血干/祖细胞抗原CD34、HLADR、髓系早期抗原CD13、CD33、单核系抗原CD14、T淋巴细胞抗原CD7的表达率增高，髓系成熟抗原CD15、B淋巴系抗原CD19、CD20的表达率降低，DI值增高（P＜0.05）；RAEB组与RA组相比CD34、HLADR、CD13、CD33、CD14、CD7的表达率增高，CD15、CD3、CD19、CD20的表达率减低，DI值增高（P＜0.05）。AA组与正常对照组相比其CD34、CD13、CD33、CD15的表达率降低，CD3、CD7、CD25、CD22的表达率增高（P＜0.05）。MDS组与AA组相比其CD34、HLADR、CD13、CD33、CD14的表达率增高，CD3、CD5、CD7、CD15、CD19、CD20、CD22、CD25的表达率显著降低（P＜0.05）。结论MDS和AA患者骨髓细胞免疫表型分析，有助于揭示二者的发病机制，为临床提供新的诊断、分型及鉴别诊断方法。

关键词: 骨髓增生异常综合征, 贫血,再生障碍性, 免疫表型分型

Abstract: To explore the role of immunophenotyping in the pathogenesis, diagnosis, classification and differential diagnosis of myelodysplastic syndrome (MDS) and aplastic anemia(AA). MethodsA panel of monoclonal antibodies and flow cytometry using CD45/SSC gating strategies were used for immunophenotyping in bone marrow nucleated cells of 23 patients with MDS, 14 patients with AA and 9 healthy controls. ResultsCompared with the control group, expressions of haemopoietic stem/progenitor cell surface markers CD34 and HLADR, early medullary system surface markers CD13 and CD33, monocyte system surface marker CD14, and T lymphocyte cell surface marker CD7 were significantly increased in the MDS group; however, expressions of later medullary system surface marker CD15, B lymphocyte cell surface markers CD19 and CD20 were significantly decreased in the MDS group（P＜0.05）. However, as RA progressed to RAEB, expressions of CD34, HLADR, CD13, CD33, CD14 and CD7 were significantly increased, while expressions of CD15, CD3, CD19 and CD20 were significantly decreased; and the DI level was significantly increased（P＜0.05）. Expression levels of CD34, CD13, CD33 and CD15 in the AA group were significantly higher than those in the control group and those of CD3, CD7, CD25 and CD22 were lower than those in the control group（P＜0.05）. Also expression levels of CD34, HLADR, CD13, CD33 and CD14 in the MDS group were significantly higher than those in the AA group, while expression levels of CD3, CD5, CD7, CD15, CD19, CD20, CD22 and CD25 were significantly lower in the MDS group than those in the AA group（P＜0.05）. ConclusionImmunophenotyping analysis may be a useful tool for the diagnosis, classification and differential diagnosis of MDS and AA.

Key words: Myelodysplastic syndrome, Anemia,aplastic, Immunophenotyping

中图分类号:

R551.3

赵广玲1 ，许洪志1 ，黄敏1 ,李元堂2 ，武焕玲2 ，李建峰3 ，马春燕3
. 骨髓增生异常综合征和再生障碍性贫血患者
骨髓原始细胞免疫表型分析[J]. 山东大学学报(医学版), 2009, 47(02): 53-57.

ZHAO Guangling¹, XU Hongzhi¹, HUANG Min¹, LI Yuantang²,
WU Huanling², LI Jianfeng³, MA Chunyan³
. Immunophenotyping in myelodysplastic syndrome and aplastic anemia
[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(02): 53-57.

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