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山东大学学报(医学版) ›› 2209, Vol. 47 ›› Issue (6): 65-.

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脑膜瘤中PPAR-γ的表达及曲格列酮对脑膜瘤培养细胞生长的影响

鹿向东 杨伟 徐广明 曲元明   

  1. 山东大学附属省立医院神经外科, 济南 250021
  • 收稿日期:2009-02-07 出版日期:2009-06-16 发布日期:2209-06-25
  • 通讯作者: 徐广明(1963- ),主任医师,主要从事微侵袭神经外科研究。 E-mail:guangmingxu@126.com
  • 作者简介:鹿向东(1981- ),男,硕士研究生,主要从事颅脑肿瘤研究。

Expression and role of PPAR-γin meningiomas and troglitazone induced meningiomas cell apoptosis in vitro

 LU Xiang-Dong, YANG Wei, XU Guang-Meng, QU Yuan-Ming   

  1. Department of Neurosurgery, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China
  • Received:2009-02-07 Online:2009-06-16 Published:2209-06-25

摘要:

目的 探讨PPAR-γ在脑膜瘤中的基因和蛋白表达以及其激动剂可能的抗肿瘤作用。方法  应用RT-PCR、免疫组化方法对48例脑膜瘤的手术标本进行PPAR-γ的基因和蛋白表达分析,并体外培养16例人脑膜瘤细胞,应用MTT方法探讨曲格列酮对脑膜瘤细胞生长的作用,应用流式细胞仪检测曲格列酮干预后凋亡情况。结果 PPAR-γ在48例脑膜瘤细胞中均有表达,且非典型组高于良性组(P<0.05),女性患者PPAR-γ基因表达高于男性;其表达与脑膜瘤增殖性正相关,曲格列酮体外抑制脑膜瘤细胞生长,并可以诱导脑膜瘤细胞凋亡,其作用具有时间和浓度的依赖性。结论 非典型脑膜瘤PPAR-γ表达高于良性组,其表达与增殖活性成正相关;其配体活化后体外可以抑制脑膜瘤细胞生长并可诱导凋亡。

关键词: 脑膜肿瘤:过氧化物酶体增生物激活受体-γ;曲格列酮:细胞凋亡

Abstract:

Objective To explore mRNA and protein expressions of PPAR-γ in meningiomas and investigate the synergistic anticancer effect of its activator in meningiomas. Methods Gene expression of PPAR-γ in 48 surgical specimens(28 benign cases and 20 atypical cases)of meningiomas was determined using RT-PCR and protein expression using an immunohisto-chemical method. Human meningioma(16 cases) cells were cultured in vitro in order to study the effect of troglitazone anticancer therapy by MTT. Flow cytometry was applied to evaluate the apoptotic rate of cultured cells. Results  Compared with the atypical group, gene and protein expressions of PPAR-γ in the benign group were decreased. Gene expression in females was stronger than in males. RT-PCR result demonstrated that growth of primary meningioma cells could be significantly inhibited by troglitazone in a concentration- and time-dependent manner. Flow cytometry results also suggested that troglitazone induced meningioma cell apoptosis in vitro and the effect was significantly increased with an increase of concentration and time. Conclusion  PPAR-γ may play an important role in the development of meningiomas. PPAR-γ agonists induce growth arrest and apoptosis in meningioma cells in vitro.

Key words: Meningiomas; Peroxisome proliferator-activated receptor gamma; Troglitazone; Apoptosis

中图分类号: 

  • R739.45
[1] 鹿向东 杨伟 徐广明 曲元明. 脑膜瘤中PPAR-γ的表达及曲格列酮对脑膜瘤培养细胞生长的影响[J]. 山东大学学报(医学版), 2009, 47(6): 65-.
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