山东大学学报（医学版） ›› 2016, Vol. 54 ›› Issue (10): 34-39.doi: 10.6040/j.issn.1671-7554.0.2015.1092
薛莹,张超,梁婷,宋静,侯桂华
XUE Ying, ZHANG Chao, LIANG Ting, SONG Jing, HOU Guihua
摘要： 目的 探讨放射性碘125标记基因重组鞭毛蛋白(125I-rFlic)及其片段(125I-rFlicΔ180-400)在同种移植模型中的生物学分布及靶向性。 方法 用1,3,4,6-四氯-3α,6α-二苯基甘脲(Iodogen)碘化标记rFlic及rFlicΔ180-400,分析标记率及稳定性,进行放射性配基结合分析;构建小鼠同种/同系皮肤移植模型,于术后第8天尾静脉注射标记物,分析生物学分布和药代动力学特征,进行全身磷屏自显影显像。 结果 成功制备125I-rFlic及rFlicΔ180-400,且具有较好的体外稳定性;125I-rFlicΔ180-400对移植受体脾细胞亲和力更高。生物学分布结果显示,与125I-rFlic组相比,125I-rFlicΔ180-400注射组24 h的 移植皮片/对侧正常皮片(T/NT)明显升高,P=0.014 8。药代动力学结果表明,与125I-rFlic相比,125I-rFlicΔ180-400代谢更快。全身磷屏自显影显像显示,注射后6 h的同种移植皮片放射性浓聚较1 h更明显;与125I-rFlic组相比,125I-rFlicΔ180-400组移植皮片显像更加清晰。注射后24 h两组均可得到清晰移植皮片显像。 结论 125I-rFlic与125I-rFlicΔ180-400在移植皮片处高度特异性浓聚,可成功显示同种移植急性排斥,而后者比前者拥有更快的代谢率和更快的特异性浓聚。
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