胰高血糖素样肽-1对高脂饲养大鼠卵巢生物钟节律的影响

doi:10.6040/j.issn.1671-7554.0.2014.533

摘要/Abstract

摘要： 目的观察胰高血糖素样肽-1(GLP-1)对高脂饲养大鼠受损卵巢生物钟的治疗作用.方法 Wistar大鼠随机分为普通饲养组、高脂饲养组及高脂饲养+GLP-1干预组;8周后测空腹血糖、胰岛素、胆固醇、甘油三酯、雌二醇、卵泡刺激素、黄体生成素的水平;实时定量PCR检测卵巢相关基因及生物钟基因的表达.结果 GLP-1对高脂饲养大鼠血清生化指标产生一定影响.与普通饲养组相比,高脂饲养组大鼠始基卵泡募集因子抗苗勒氏管激素(AMH)、生长分化因子-9(GDF-9)的表达水平降低(P<0.05),而高脂饲养GLP-1干预组AMH、GDF-9的mRNA的表达明显改善.此外,与普通饲养组相比,高脂饲养组大鼠卵巢生物钟相关基因的表达具有明显变化,而GLP-1可改善高脂饲养对卵巢生物钟相关基因表达的影响.结论 GLP-1对高脂饲养所致的卵巢生物钟受损具有改善作用.

关键词: 大鼠, 高脂饲养, 生物钟, 卵巢, 胰高血糖素样肽-1

Abstract: Objective To explore the therapeutic effect of glucagon-like peptide-1(GLP-1) on impaired circadian gene of ovary in rats fed with high-fat diet. Methods Wistar rats were randomly divided into control group, high-fat diet group and GLP-1 intervention group. Eight weeks later, the levels of fasting glucose, insulin, cholesterol, triglyceride, E2, FSH and LH were tested. The expression of ovary related gene and circadian gene in ovary tissues were detected with Real-time PCR. Results GLP-1 had certain effect on serum biochemical indexes in rats fed with high fat. Real-time PCR results showed that, compared with control group, the levels of primordial follicle recruitment factor anti-mullerian homone (AMH) and growth differentiation factor (GDF-9) were significantly decreased in rats fed with high-fat diet (P<0.05). However, the expression of AMH and GDF-9 mRNA in high-fat diet rats treated with GLP-1 were markedly elevated. Besides, compared with control group, the expression of ovary circadian clock related genes was affected in rats fed with high-fat diet, and GLP-1 could improve the expression of ovary circadian gene. Conclusion GLP-1 could ameliorate the expression of ovary circadian gene in rats fed with high-fat diet.

Key words: Circadian clock, High-fat feeding, Glucagon-like peptide-1, Ovary, Rats

中图分类号:

R711.6

姚志娜, 韩婷, 范秀玲, 章由生, 宋佳伦. 胰高血糖素样肽-1对高脂饲养大鼠卵巢生物钟节律的影响[J]. 山东大学学报（医学版）, 2015, 53(2): 19-21.

YAO Zhina, HAN Ting, FAN Xiuling, ZHANG Yousheng, SONG Jialun. Effect of Glucagon-like peptide-1 on ovarian circadian clock rhythm of rats fed with high fat diet[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(2): 19-21.

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