miRNA-206在胃间质瘤组织中的表达及其临床意义

doi:10.6040/j.issn.1671-7554.0.2015.172

山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (12): 67-70.doi: 10.6040/j.issn.1671-7554.0.2015.172

miRNA-206在胃间质瘤组织中的表达及其临床意义

姚冬雪, 赵琪, 秦成勇

山东大学附属省立医院消化内科, 山东济南 250021

收稿日期:2015-02-10 出版日期:2015-12-10 发布日期:2015-12-10
通讯作者: 秦成勇。E-mail:qinchengyongls@163.com E-mail:qinchengyongls@163.com
基金资助:
国家自然科学基金(81472685)

Clinical significance of miRNA-206 expression in gastric stromal tumor

YAO Dongxue, ZHAO Qi, QIN Chengyong

Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China

Received:2015-02-10 Online:2015-12-10 Published:2015-12-10

摘要/Abstract

摘要： 目的探讨miRNA-206在胃间质瘤中的表达及意义。方法收集胃间质瘤治疗后的切除标本,采用qRT-PCR方法检测53例胃间质瘤及对应正常胃组织中miRNA-206的表达,并分析其与临床病理因素的关系。结果胃间质瘤组织中miRNA-206表达显著低于对应正常胃组织(P<0.05),且核分裂数 ≥ 5/50 HPF病例的miRNA-206表达显著低于核分裂数<5/50 HPF病例的miRNA-206表达(P<0.05), Fletcher分级中/高危病例的miRNA-206表达显著低于极低/低危分级病例的miRNA-206表达(P<0.05)。结论 miRNA-206在胃间质瘤组织中表达下调,且与核分裂像及Fletcher分级密切相关,提示其在胃间质瘤的发生发展过程中发挥重要作用。

关键词: 胃间质肿瘤, miRNA-206, 核分裂像, 临床特征, Fletcher分级

Abstract: Objective To evaluate the expression and significance of miRNA-206 in the development of gastric stromal tumor. Methods Fifty-three cases of the surgical specimens of gastric stromal tumor were collected. miRNA-206 levels in matched pairs of gastric stromal tumor and normal adjacent tissue samples were examined using qRT-PCR; the relationship between miRNA-206 levels and clinical characteristics were investigated. Results Expression of miRNA-206 was significantly lower in tumor than that in adjacent normal tissues (P<0.05). Expression of miRNA-206 in cases with mitotic count ≥ 5/50HPF was significantly lower than that with mitotic count<5/50HPF (P<0.05). By Fletcher risk stratification system, expression of miRNA-206 in intermediate/high-risk cases was significantly lower than that in very low/low risk cases (P<0.05). Conclusion In gastric stromal tumor, miRNA-206 expression is significantly down-regulated, and is closely associated with mitotic counts and Fletcher risk stratification system, which suggests that miRNA-206 play an important role in the process of development of gastric stromal tumor.

Key words: Gastric stromal tumors, Clinical characteristics, miRNA-206, Mitotic count, Fletcher risk stratification

中图分类号:

R573

姚冬雪, 赵琪, 秦成勇. miRNA-206在胃间质瘤组织中的表达及其临床意义[J]. 山东大学学报（医学版）, 2015, 53(12): 67-70.

YAO Dongxue, ZHAO Qi, QIN Chengyong. Clinical significance of miRNA-206 expression in gastric stromal tumor[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(12): 67-70.

参考文献

[1] Shinomura Y, Kinoshita K, Tsutsui S, et al. Pathophysiology, diagnosis, and treatment of gastrointestinal stromal tumors[J]. J Gastroenterol, 2005, 40(8):775-780.
[2] Corless CL, Fletcher JA, Heinrich MC. Biology of gastrointestinal stromal tumors[J]. J Clin Oncol, 2004, 22(18):3813-3825.
[3] He L, Hannon GJ. MicroRNAs:small RNAs with a big role in gene regulation[J]. Nat Rev Genet, 2004, 5(7):522-531.
[4] Croce CM. Causes and consequences of microRNA dysregulation in cancer[J]. Nat Rev Genet, 2009, 10(10):704-714.
[5] Fletcher CD, Berman JJ, Corless C, et al. Diagnosis of gastrointestinal stromal tumors:a consensus approach[J]. Int J Surg Pathol, 2002, 10(2):81-89.
[6] Miettinen M, Lasota J. Gastrointestinal stromal tumors:review on morphology, molecular pathology, prognosis, and differential diagnosis[J]. Arch Pathol Lab Med, 2006, 130(10):1466-1478.
[7] Lennartsson J, Ronnstrand L. The stem cell factor receptor/c-Kit as a drug target in cancer[J]. Curr Cancer Drug Targets, 2006, 6(1):65-75.
[8] Fletcher CD, Berman JJ, Corless C, et al. Diagnosis of gastrointestinal stromal tumors:a consensus approach[J]. Hum Pathol, 2002, 33(5):459-465.
[9] Subramanian S, Lui WO, Lee CH, et al. MicroRNA expression signature of human sarcomas[J]. Oncogene, 2008, 27(14):2015-2026.
[10] Jovanovic M, Hengartner MO. miRNAs and apoptosis:RNAs to die for[J]. Oncogene, 2006, 25(46):6176-6187.
[11] Fabian MR, Sonenberg N. The mechanics of miRNA-mediated gene silencing:a look under the hood of miRISC[J]. Nat Struct Mol Biol, 2012, 19(6):586-593.
[12] Wang X, Ling C, Bai Y, et al. MicroRNA-206 is associated with invasion and metastasis of lung cancer[J]. Anat Rec (Hoboken), 2011, 294(1):88-92.
[13] Zhang L, Liu X, Jin H, et al. miR-206 inhibits gastric cancer proliferation in part by repressing cyclinD2[J]. Cancer Lett, 2013, 332(1):94-101.
[14] Li Y, Hong F, Yu Z. Decreased expression of microRNA-206 in breast cancer and its association with disease characteristics and patient survival[J]. J Int Med Res, 2013, 41(3):596-602.
[15] Zhou J, Tian Y, Li J, et al. miR-206 is down-regulated in breast cancer and inhibits cell proliferation through the up-regulation of cyclinD2[J]. Biochem Biophys Res Commu, 2013, 433(2):207-212.
[16] Zhang T, Liu M, Wang C, et al. Down-regulation of MiR-206 promotes proliferation and invasion of laryngeal cancer by regulating VEGF expression[J]. Anticancer Res, 2011, 31(11):3859-3863.
[17] Taulli R, Bersani F, Foglizzo V, et al. The muscle-specific microRNA miR-206 blocks human rhabdomyosarcoma growth in xenotransplanted mice by promoting myogenic differentiation[J]. J Clin Invest, 2009, 119(8):2366-2378.
[18] Liu W, Xu C, Wan H, et al. MicroRNA-206 overexpression promotes apoptosis, induces cell cycle arrest and inhibits the migration of human hepatocellular carcinoma HepG2 cells[J]. Int J Mol Med, 2014, 34(2):420-428.
[19] Bao YP, Yi Y, Peng LL, et al. Roles of microRNA-206 in osteosarcoma pathogenesis and progression[J]. Asian Pac J Cancer Prev, 2013, 14(6):3751-3755.
[20] Haller F, von Heydebreck A, Zhang JD, et al. Localization-and mutation-dependent microRNA (miRNA) expression signatures in gastrointestinal stromal tumours (GISTs), with a cluster of co-expressed miRNAs located at 14q32.31[J]. J Pathol, 2010, 220(1):71-86.
[21] Yamamoto H, Kohashi K, Fujita A, et al. Fascin-1 overexpression and miR-133b downregulation in the progression of gastrointestinal stromal tumor[J]. Mod Pathol, 2013, 26(4):563-571.
[22] Niinuma T, Suzuki H, Nojima M, et al. Upregulation of miR-196a and HOTAIR drive malignant character in gastrointestinal stromal tumors[J]. Cancer Res, 2012, 72(5):1126-1136.
[23] 刘志,韩少良,黄颖鹏,等.miR-143和miR-145在胃间质瘤组织中的表达及其意义[J].中华普通外科杂志, 2010, 25(8):678-680. LIU Zhi, HAN Shaoliang, HUANG Yingpeng, et al. Clinical significance of miR-143 and miR-145 expression in gastric gastrointestinal stromal tumor[J]. Chin J Gen Surg, 2010, 25(8):678-680.
[24] Liu S, Cui J, Liao G, et al. MiR-137 regulates epithelial-mesenchymal transition in gastrointestinal stromal tumor[J]. Tumour Biol, 2014, 35(9):9131-9138.

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miRNA-206在胃间质瘤组织中的表达及其临床意义

Clinical significance of miRNA-206 expression in gastric stromal tumor

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