下肢静脉曲张患者外周血Nelin水平测定及意义

山东大学学报(医学版) ›› 2013, Vol. 51 ›› Issue (9): 64-66.

下肢静脉曲张患者外周血Nelin水平测定及意义

裴长安，秦士勇，陈士辉，张曙光

山东大学附属千佛山医院血管外科，济南 250014

收稿日期:2012-10-29 出版日期:2013-09-10 发布日期:2013-09-10
通讯作者: 张曙光， E-mail：shuguang828@163.com
基金资助:
山东省自然科学基金(ZR2009CL018)

Detection of the Nelin in peripheral blood of patients with varicose veins of lower extremity and its significance

PEI Chang-an, QIN Shi-yong, CHEN Shi-hui, ZHANG Shu-guang

Department of Vascular Surgery, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, China

Received:2012-10-29 Online:2013-09-10 Published:2013-09-10

摘要/Abstract

摘要：

目的探讨下肢静脉曲张患者与正常人外周血中Nelin表达水平的差异及其意义。方法选取下肢静脉曲张患者57例为对照组，健康查体者60例为正常组，利用RT-PCR技术检测外周血单个核细胞中Nelin mRNA的表达水平，利用生物素双抗体夹心ELISA技术测定外周血血清中Nelin蛋白的表达水平。结果 RT-PCR结果显示，Nelin在对照组与正常组的相对表达量分别是 0.226±0.070、0.718±0.114，两组差异有统计学意义(t=9.028，P<0.01)；ELISA结果显示，96例血清中均可检测到Nelin蛋白的表达，对照组和正常组的表达量分别是(2.61±1.73)ng/mL、(3.64±1.65)ng/mL，两组差异有统计学意义(t=2.92，P<0.01)。结论与正常人相比，下肢静脉曲张患者Nelin基因在转录和翻译水平均存在明显的表达下调，提示Nelin基因的表达可能与下肢静脉曲张发生发展有一定的关系。

关键词: 静脉曲张；Nelin；基因表达；表型转化

Abstract:

Objective To explore the different expressions and significance of Nelin in the peripheral blood of healthy controls and patients with varicose veins of lower extremity. Methods A total of 57 patients with varicose veins of lower extremity treated in our hospital and 60 healthy controls were enrolled in the study. The expression of Nelin mRNA in the peripheral blood was detected with RT-PCR; Nelin protein in the peripheral blood was determined with double-antibody sandwich ELISA technique. Results The results of RT-PCR showed that the expressions of Nelin mRNA in patients and controls were 0.226±0.070 and 0.718±0.114 respectively (t=9.028, P<0.01), with statistical difference. ELISA examination showed that Nelin protein was detectable in all serum samples, which was (2.61±1.73) ng/mL in patients and (3.64±1.65)ng/mL in controls (t=2.92, P<0.01). The difference was statistically significant. Conclusion The expression of Nelin in patients with varicose veins is significantly lower than that in healthy controls at both transcription and translation levels, indicating that the expression of Nelin is related to the pathogenesis and development of varicose veins of lower extremity.

Key words: Varicose veins; Nelin; Gene expression; Phenotypic modulation

中图分类号:

静脉曲张

裴长安，秦士勇，陈士辉，张曙光. 下肢静脉曲张患者外周血Nelin水平测定及意义[J]. 山东大学学报(医学版), 2013, 51(9): 64-66.

PEI Chang-an, QIN Shi-yong, CHEN Shi-hui, ZHANG Shu-guang. Detection of the Nelin in peripheral blood of patients with varicose veins of lower extremity and its significance[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(9): 64-66.

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