慢病毒载体靶向携带survivin-siRNA对恶性黑色素瘤A375细胞的影响

山东大学学报(医学版) ›› 2013, Vol. 51 ›› Issue (5): 54-57.

慢病毒载体靶向携带survivin-siRNA对恶性黑色素瘤A375细胞的影响

朱守荣1，张芮2，曹永倩1，冯璋1，王一兵1

1.山东大学附属省立医院烧伤整形科，济南 250021；
2.山东医学高等专科学校病理与病理生理教研室，济南 250002

收稿日期:2012-08-09 出版日期:2013-05-10 发布日期:2013-05-10
通讯作者: 王一兵, E-mail：wyb0616@yahoo.com.cn
基金资助:
山东省科技攻关计划(2007GG20002044)

The effect of the survivin-siRNA targeting lentivirus vector on human malignant melanoma cell line A375

ZHU Shou-rong1， ZHANG Rui2， CAO Yong-qian1， FENG Zhang1， WANG Yi-bing1

1. Burn and Plastic Surgery， Provincial Hospital Affiliated to Shandong University， Jinan 250021， China；
2. Department of Pathology and Pathophysiology， Shandong Medical Collegy， Jinan 250002， China

Received:2012-08-09 Online:2013-05-10 Published:2013-05-10

摘要/Abstract

摘要：

目的观察靶向survivin的RNA干扰对人恶性黑色素瘤A375细胞的影响。方法将处于对数生长期的黑色素瘤A375细胞随机分为3组：阳性组、阴性对照组、空白对照组，将设计并构建好的survivin-siRNA慢病毒载体分别转染入各组细胞内，实时定量PCR及Western-blot法分别检测转染前后3组细胞survivin mRNA及蛋白的表达情况。结果成功设计并构建了survivin-siRNA慢病毒载体，与空白对照组相比，转染survivin-siRNA的阳性组survivin mRNA及蛋白的表达明显降低，抑制率分别为60%、87.38%，差异有统计学意义(P＜0.05)。结论靶向survivin的siRNA能够明显抑制恶性黑色素瘤A375细胞的survivin基因的表达。

关键词: 恶性黑色素瘤；survivin基因；RNA干扰；慢病毒载体

Abstract:

Objective To observe the effect of the RNA interference (RNAi) targeting survivin on human malignant melanoma cell line A375. Methods The human malignant melanoma cell line A375 was divided into there groups: the survivin-siRNA LV group, the NC-GFP-LV group and the control group. After designing and constructing the survivin-siRNA lentivirus vector, transfect it into these there groups of cells. The expression of survivin mRNA and protein were detected by real time-PCR and Western blot respectively. Results The survivin-siRNA lentivirus vector was designed and constructed successfully. After the transfection, the expressions of survivin mRNA and protein were significantly lower in the survivin-siRNA LV group than those in the control group(P<0.05), with the inhibition ratio of 60% and 87.38%. Conclusion The shRNA targeting survivin can down-regulate the expression of survivin gene in melanoma A375 cells.

Key words: Malignant melanoma； Survivin gene； RNA interference； Lentivirus vector

中图分类号:

R730.5

朱守荣1，张芮2，曹永倩1，冯璋1，王一兵1. 慢病毒载体靶向携带survivin-siRNA对恶性黑色素瘤A375细胞的影响[J]. 山东大学学报(医学版), 2013, 51(5): 54-57.

ZHU Shou-rong1， ZHANG Rui2， CAO Yong-qian1， FENG Zhang1， WANG Yi-bing1. The effect of the survivin-siRNA targeting lentivirus vector on human malignant melanoma cell line A375[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(5): 54-57.

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