健脾益肾、活血化瘀中药复方对高糖刺激下肾小球系膜细胞增殖的影响

山东大学学报(医学版) ›› 2013, Vol. 51 ›› Issue (5): 29-32.

健脾益肾、活血化瘀中药复方对高糖刺激下肾小球系膜细胞增殖的影响

刘丹丹1,高聆2，赵家军1，韩文霞1

1.山东大学附属省立医院内分泌科，山东省内分泌代谢病临床医学中心，
山东省临床医学研究院内分泌与代谢研究所, 济南 250021；
2.山东大学附属省立医院中心实验室, 济南 250021

收稿日期:2013-01-08 出版日期:2013-05-10 发布日期:2013-05-10
通讯作者: 韩文霞， E-mail：hanwx97@163.com
基金资助:
国家自然科学基金(30701130)

Effect of Jianpiyishen-Huoxuehuayu Chinese herbal compound prescription on renal mesangial cells proliferation in high glucose

LIU Dan-dan1， GAO Ling1, ZHAO Jia-jun1, HAN Wen-xia1

1. Department of Endocrinology, Provincial Hospital Affiliated to Shandong University, Institute of Endocrinology,
Shandong Clinical Medicine Academy, Shandong Clinical Medicine Center of Endocrinology, Jinan 250021;
2. Central Laboratory of Provincial Hospital Affiliated to Shandong University, Jinan 250021, China

Received:2013-01-08 Online:2013-05-10 Published:2013-05-10

摘要/Abstract

摘要：

目的探讨健脾益肾、活血化瘀中药复方对高糖刺激下大鼠肾小球系膜细胞(RMCs)增殖的影响及对转化生长因子-β1(TGF-β1)和骨形态发生蛋白-7(BMP-7)表达的影响。方法将常规培养的RMCs分为对照组(5.6mmol/L葡萄糖组)、甘露醇组(5.6mmol/L葡萄糖+24.4mmol/L甘露醇)、高糖组(30mmol/L葡萄糖组)、高糖+中药复方组(30mmol/L葡萄糖+0.65mg/mL中药复方)。培养48h，采用4 -甲偶氮唑蓝(MTT)法检测细胞增殖情况，分析健脾益肾、活血化瘀中药复方对高糖刺激下RMCs增殖的影响。酶联免疫法检测TGF-β1的表达，实时定量PCR法检测BMP-7 mRNA的表达。结果高糖组RMCs增生及TGF-β1的表达较对照组增加明显，BMP-7 mRNA的表达则明显下降，高糖+中药复方组较高糖组RMCs增生及TGF-β1的表达明显下降，BMP-7 mRNA的表达明显升高。结论健脾益肾、活血化瘀中药复方能够抑制高糖刺激下的RMCs增殖及TGF-β1的表达，并上调BMP-7 mRNA的表达。

关键词: 大鼠肾小球系膜细胞；健脾益肾、活血化瘀中药复方；转化生长因子-β1；骨形态发生蛋白-7

Abstract:

Objective To study the effect of Jianpiyishen-Huoxuehuayu Chinese herbal compound prescription on renal mesangial cell proliferation and expressions of TGF-β1 and BMP-7 in high glucose. Methods RMCs of conventional culture were divided into the normal control group, mannitol group, high glucose group and high glucose with 0.65mg/mL traditional Chinese medicine group. The glucose concentration of normal group was 5.6mmol/L while 30mmol/L in the high glucose group. After 48h, cell proliferation was detected with 4 -A thiazolyl blue. TGF-β1 was detected with ELISA. BMP-7 mRNA was detected with RT-PCR. Results Compared with the blank control group, cell proliferation and expression of TGF-β1 of high glucose group were obviously increased while the level of BMP-7 mRNA was decreased. Compared with the high glucose group, cell proliferation and expression of TGF-β1 of Chinese herbal compound prescription obviously decreased while expression of BMP-7 mRNA was increased. Conclusion High glucose could promote the proliferation and expression of TGF-β1 of RMC. It also could decrease the expression of BMP-7 mRNA. Chinese herbal compound prescription could restrain the proliferation and expression of TGF-β1 of RMC in high glucose while it could increase the expression of BMP-7 mRNA.

Key words: Rat mesangial cells; Jianpiyishen-Huoxuehuayu Chinese herbal compound prescription; Tissue growth factor-β1; Bone morphogenetic protein-7

中图分类号:

R587.1

刘丹丹1,高聆2，赵家军1，韩文霞1. 健脾益肾、活血化瘀中药复方对高糖刺激下肾小球系膜细胞增殖的影响[J]. 山东大学学报(医学版), 2013, 51(5): 29-32.

LIU Dan-dan1， GAO Ling1, ZHAO Jia-jun1, HAN Wen-xia1. Effect of Jianpiyishen-Huoxuehuayu Chinese herbal compound prescription on renal mesangial cells proliferation in high glucose[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(5): 29-32.

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