促甲状腺激素对破骨细胞分化的影响

山东大学学报(医学版) ›› 2013, Vol. 51 ›› Issue (4): 6-10.

促甲状腺激素对破骨细胞分化的影响

张雯雯1，张艳玲1，王杰1，高聆2，于春晓1，阎慧丽1，徐进1

1. 山东大学附属省立医院内分泌科，山东省内分泌代谢病临床医学中心;
2. 山东大学附属省立医院中心实验室，济南 250021

收稿日期:2012-12-17 出版日期:2013-04-10 发布日期:2013-04-10
通讯作者: 徐进（1968- ），女，教授，主任医师，主要从事内分泌与代谢病研究。E-mail：xujin@medmail.com.cn
作者简介:张雯雯（1986- ），女，硕士研究生，主要从事内分泌与代谢病研究。E-mail: yixuesheng@yahoo.cn
基金资助:
山东省优秀中青年科学家科研奖励基金（2007BS03004）

Effects of thyroid stimulating hormone on osteoclast differentiation

ZHANG Wen-wen1， ZHANG Yan-ling1， WANG Jie1， GAO Ling2,YU Chun-xiao1, YAN Hui-li1， XU Jin1

1. Department of Endocrinology, Provincial Hospital Affiliated to Shandong University,
Shandong Clinical Medical Center of Endocrinology and Metabolism;
2. Central Laboratory, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China

Received:2012-12-17 Online:2013-04-10 Published:2013-04-10

摘要/Abstract

摘要：

目的探讨促甲状腺激素（TSH）对小鼠单核/巨噬细胞系RAW264.7细胞诱导分化为破骨样细胞过程的影响。方法体外培养小鼠单核/巨噬细胞系RAW264.7细胞，以破骨细胞分化因子 (RANKL) 诱导分化，同时加用不同浓度TSH处理7d，抗酒石酸酸性磷酸酶（TRAP）染色鉴定并计数阳性细胞数量，采用Real-time PCR方法检测分化标志蛋白TRAP、基质金属蛋白酶-9（MMP-9）及组织蛋白酶K（Cathepsin K）的基因表达。结果 RAW264.7细胞可在RANKL诱导下分化为破骨样细胞，高表达TRAP、MMP-9和Cathepsin K基因，TSH可剂量依赖性抑制细胞分化并下调上述基因的表达。结论 TSH可抑制破骨细胞的分化，对于维持骨量可能具有重要作用。

关键词: 促甲状腺激素；RAW264.7细胞；破骨细胞；分化

Abstract:

Objective To study the effects of thyroid stimulating hormone (TSH) on the differentiation of RAW264.7 cells into osteoclast like cells. Methods RAW264.7 cells were induced to differentiate into osteoclasts by addition of ligand of receptor activator of NF-κB (RANKL), and treated with different doses of TSH for 7 days. Tartrate resistant acid phosphatase (TRAP) staining was used to identify and count osteoclasts. The mRNA expression of osteoclastic phenotypes and functioning genes including TRAP, matrix metalloproteinase-9 (MMP-9) and Cathepsin K in different groups were detected by Real-time PCR. Results RAW264.7 cells could be induced to differentiate into mature osteoclasts and express high levels of TRAP, MMP-9 and Cathepsin K by RANKL. TSH dose-dependently inhibited the cell differentiation and down-regulated the expression of the genes above including TRAP, MMP-9 and Cathepsin K. Conclusion TSH negatively regulates osteoclast differentiation, which may contribute to the maintenance of bone mass.

Key words: Thyroid stimulating hormone； RAW264.7 cells； Osteoclast； Differentiation

中图分类号:

R581.1

张雯雯1，张艳玲1，王杰1，高聆2，于春晓1，阎慧丽1，徐进1. 促甲状腺激素对破骨细胞分化的影响[J]. 山东大学学报(医学版), 2013, 51(4): 6-10.

ZHANG Wen-wen1， ZHANG Yan-ling1， WANG Jie1， GAO Ling2,YU Chun-xiao1, YAN Hui-li1， XU Jin1. Effects of thyroid stimulating hormone on osteoclast differentiation[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(4): 6-10.

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