新骨架小分子南强菌素对紫杉醇耐药性的逆转作用及其机制

山东大学学报(医学版) ›› 2013, Vol. 51 ›› Issue (4): 21-25.

新骨架小分子南强菌素对紫杉醇耐药性的逆转作用及其机制

张成梅1，武传涛1，朱珊珊2，贾舒3，王一4，史艳秋1,2

1.山东大学实验动物中心，济南 250012； 2.山东大学药学院，济南 250012；
3.山东大学生命科学学院，济南 250010； 4.山东西王集团有限公司，山东邹平 256200

收稿日期:2012-10-18 出版日期:2013-04-10 发布日期:2013-04-10
通讯作者: 史艳秋(1966- )，女，博士，副教授，主要从事肿瘤药理学研究。 E-mail:shiyq@sdu.edu.cn
作者简介:张成梅(1967- )，女，硕士，副教授，主要从事药物毒理学研究。 E-mail：zhangcm@sdu.edu.cn
基金资助:
国家自然科学基金面上项目(81072569)；国家自然科学基金青年项目(31000368)；山东省自然科学基金面上项目(ZR2010HM088)

Effect and mechanism of deacetyl-mycoepoxydiene on reversing resistance to taxol in tumor cells

ZHANG Cheng-mei1， WU Chuan-tao1， ZHU Shan-shan2， JIA Shu3， WANG Yi4， Shi Yan-qiu1,2

1. Experimental Animal Center Shandong University， Jinan 250012, China；
2. School of Pharmaceutical Sciences， Shandong University, Jinan 250012, China；
3. School of Life Science, Shandong University, Jinan 250010, China； 4. Shandong Xiwang Group Company,
Zouping 256200, Shandong, China

Received:2012-10-18 Online:2013-04-10 Published:2013-04-10

摘要/Abstract

摘要：

目的研究南强菌素(DM)对肿瘤细胞紫杉醇耐药性的逆转作用并探讨其逆转机制。方法 MTT法检测南强菌素对A549/T耐药细胞与A549敏感细胞的毒性及其对紫杉醇敏感性的影响，以Annexin V法检测细胞凋亡率和Rh123在耐药细胞中的积累，HPLC测定细胞内紫杉醇的蓄积量，Western blotting检测Ⅲ型β微管蛋白的表达水平。结果南强菌素可显著提高A549/T细胞对紫杉醇的敏感性，增加紫杉醇诱导耐药细胞凋亡率，增强A549/T细胞中紫杉醇和Rh123的蓄积能力，同时，亦可明显抑制A549/T细胞中Ⅲ型β微管蛋白的表达水平。结论南强菌素可有效提高耐药细胞对紫杉醇的敏感性，逆转A549/T细胞对紫杉醇的耐药作用，其逆转机制可能与南强菌素抑制耐药肿瘤细胞膜上耐药蛋白P-糖蛋白的外泵转运功能和干扰Ⅲ型β微管蛋白的表达水平有关。

关键词: 南强菌素；紫杉醇；肿瘤细胞；多药耐药性；A549

Abstract:

Objective To investigate the effect and mechanism of deacetyl-mycoepoxydiene (DM) on reversing resistance to taxol in tumor cells. Methods MTT colorimetric assay was employed to evaluate the cytotoxicity of DM to sensitive tumor cells A549 and drug resistant cells A549/T. The influence of DM on cellular taxol sensitivity was analyzed as well. The reversal fold was approximated by MTT, and the apoptotic percentage was estimated in AnnexinV method. The accumulation of Rh123 in drug-resistance cells was observed by flow cell cytometry (FCM). Additionally, the content of taxol and the expression of Ⅲ-β-tubulin protein were respectively quantified by high performance liquid chromatography (HPLC) and Western blotting. Results This study demonstrated that DM enhanced the cellular taxol sensitivity and thus increased the apoptotic percentage of taxol-resistant tumor cells. The content of taxol and Rh123 was greatly increased in DM-treated A549/T cells. Moreover,Western blotting results showed that DM inhibited the expression of Ⅲ-β-tubulin protein in A549/T cells. Conclusion DM can effectively reverse the multidrug resistance and increase the drug sensitivity of tumor cells, which may function by interfering the expression of Ⅲ-β-tubulin protien and/or inhibiting the drug-resistant P-glyco-protein-mediated efflux pump on the cell membrane.

Key words: Deacetyl-mycoepoxydiene； Taxol； Tumor cells； Multidrug resistance; A549

中图分类号:

R965

张成梅1，武传涛1，朱珊珊2，贾舒3，王一4，史艳秋1,2. 新骨架小分子南强菌素对紫杉醇耐药性的逆转作用及其机制[J]. 山东大学学报(医学版), 2013, 51(4): 21-25.

ZHANG Cheng-mei1， WU Chuan-tao1， ZHU Shan-shan2， JIA Shu3， WANG Yi4， Shi Yan-qiu1,2. Effect and mechanism of deacetyl-mycoepoxydiene on reversing resistance to taxol in tumor cells[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(4): 21-25.

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