关键词: 帕金森病；纹状体；定向注射；1-三氯甲基-l，2，3，4-四氢化-β-咔啉；TaClo；三氯乙烯；黑质；Cox-2；大鼠，Wistar

Abstract:

Objective  To study Wistar rats’ neurobehavioral changes and cox-2 expression in substantia nigra induced by TaClo striatum stereotactic injection. Methods  Stereotactic injection technology was adopted to inject PEG dissolved TaClo to the striatum of rats. The rats of control group received the polyethylene glycol injection. At the 7th, 14th, 21st and 28th days after the injection, two groups’ mean amount of drinking water was measured. The total time, moving distance and tracks involved in the rats’ activity of the two groups were also recorded and immunohistochemical staining. Results  PD behaviors began to appear gradually among the TaClo striatum stereotactic injected rats in the experimental group. The mean amount of drinking water was reduced. Water amount of daily drinking in the experimental group was less than that in the control group (t=4.83, P<0.05). Mean distance involved in the rats’ activity of the experimental group was obviously less than that of the control group (t=14.32, P<0.01). Mean time involved in the rats’ activity of the two groups showed no difference. Motion tracks showed that, with time going on, the rats’ activities in the experimental group became less, and the active region trended to reduce to the center area. Cox-2 expression in substantia nigra was positive in histochemical staining. Conclusion  TaClo, one metabolite of the environmental neurotoxin TCE, could lead to the neurobehavioral changes of PD. Its mechanism is to cause inflammatory reaction by cox-2 mediated substantia nigra, which induces PD.

Key words: Parkinson disease; Striatum; Stereotactic injection; 1-trichloromethyl-1,2,3,4-tetrahydro-beta-carboline; TaClo; Trichloroethylene; Substantia nigra; Cox-2; Rats， Wistar