片叶苔素D大鼠体内代谢产物的结构鉴定

山东大学学报(医学版) ›› 2013, Vol. 51 ›› Issue (11): 56-60.

片叶苔素D大鼠体内代谢产物的结构鉴定

凌霄1,2，郭东晓2，娄红祥1

1.山东大学药学院天然药物化学研究所，济南 250012； 2.山东省食品药品检验所，济南 250101

收稿日期:2013-06-28 出版日期:2013-11-10 发布日期:2013-11-10
通讯作者: 娄红祥， E-mail:louhongxiang@sdu.edu.cn
基金资助:
国家自然科学基金(30730109)；国家杰出青年科学基金(30925038)

Structural determination of riccardin D′s metabolites in rats

LING Xiao1,2, GUO Dong-xiao2, LOU Hong-xiang1

1. Department of Natural Product Chemistry, School of Pharmaceutical Sciences,
Shandong University, Jinan 250012, China;
2. Institute for Food and Drug Control of Shandong Province, Jinan 250101, China

Received:2013-06-28 Online:2013-11-10 Published:2013-11-10

摘要/Abstract

摘要：

目的研究片叶苔素D在大鼠体内的代谢，并对片叶苔素D在大鼠体内代谢产物进行分离与结构鉴定。方法大鼠按40mg/kg剂量口服片叶苔素D后收集胆汁，采用高效液相色谱仪分离、纯化代谢产物，利用HPLC-MS/MS分析代谢产物，利用NMR鉴定结构。结果大鼠口服片叶苔素D后，在胆汁中检测到2个Ⅱ相代谢产物，均为片叶苔素D与葡萄糖醛酸的结合产物(片叶苔素D-1’-O-β-D-吡喃葡萄糖醛酸)，且两个代谢产物互为旋阻异构体，室温下可互相转化。结论片叶苔素D在大鼠体内产生与葡萄糖醛酸结合的Ⅱ相代谢产物，且2个代谢产物互为旋阻异构体。

关键词: 片叶苔素D；代谢产物；大鼠

Abstract:

Objective To investigate the metabolism pathway and identify the metabolites of riccardin D in vivo after oral administration in rats. Methods Using HPLC, the metabolites were purified from rats′ bile samples after oral administration of riccardin D 40mg/kg in rats. The structures of the metabolites were identified by HPLC-MS/MS and confirmed by NMR. Results The riccardin D′s metabolites were the conjugates with glucuronate and were identified as a mixture of two atropisomers. Conclusion The riccardin D′s main metabolism pathway in vivo is phase Ⅱ metabolism and the metabolites are the conjugate with glucuronate.

Key words: Riccardin D; Metabolite; Rats

中图分类号:

R574

凌霄1,2，郭东晓2，娄红祥1. 片叶苔素D大鼠体内代谢产物的结构鉴定[J]. 山东大学学报(医学版), 2013, 51(11): 56-60.

LING Xiao1,2, GUO Dong-xiao2, LOU Hong-xiang1. Structural determination of riccardin D′s metabolites in rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(11): 56-60.

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