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山东大学学报(医学版) ›› 2013, Vol. 51 ›› Issue (1): 12-.

• 基础医学 • 上一篇    下一篇

芩丹胶囊对自发性高血压大鼠心室肥厚的抑制

闫磊1，张继东1，黄山英2，王博1，任敏1，乔云1，吕怡静1，郭雪峰1   

  1. 山东大学齐鲁医院   1.中医科； 2.  教育部和卫生部心血管重构和功能研究重点实验室，济南 250012
  • 收稿日期:2012-07-05 出版日期:2013-01-10 发布日期:2013-01-10
  • 通讯作者: 张继东(1949- )，教授，博士生导师，主要从事中西医结合治疗心血管病的研究。E-mail:drzjd@sdu.edu.cn
  • 作者简介:闫磊(1979- )，男，博士研究生，主要从事中西医结合心血管病研究。E-mail:wangyanjie-119@163.com
  • 基金资助:

    国家自然科学基金(30873324)；山东省科技发展计划（2010GWZ20242）

Qin Dan capsule inhibits ventricular hypertrophy in spontaneously hypertensive rats

YAN  Lei1,  ZHANG Ji-dong1, HUANG Shan-ying2， WANG Bo1, REN Min1,QIAO Yun1, L Yi-jing1，  GUO Xue-feng1   

  1. 1. Department of Traditional Chinese Medicine； 2. Κey Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Ministry of public Health, Qilu hospital of Shandong University,  Jinan 250012, China
  • Received:2012-07-05 Online:2013-01-10 Published:2013-01-10

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摘要：

目的   观察芩丹胶囊（QD）通过激活PPARγ抑制自发性高血压大鼠（SHR）心肌肥厚及心肌组织NF-κB的蛋白表达，探讨QD逆转高血压心室肥厚的作用机制。方法   将自发性高血压大鼠（SHR）随机分为模型组(SHR组)、QD大剂量组(SHR＋QDH组)、QD小剂量组（SHR＋QDL组）、替米沙坦组(SHR＋Tel组)，WΚY大鼠作为对照组，WKY组同时灌胃给予等量生理盐水。尾袖体表描记法测量各组大鼠收缩压；HE染色观察大鼠心肌组织的改变; 透射电镜观察心肌超微结构变化; 免疫组化法观察PPARγ和NF-κB蛋白在心肌组织的表达。结果   与SHR组相比, SHR＋QDH组、SHR＋QDL组和SHR＋Tel组收缩压均下降（P<0.05）；在心肌组织中，PPARγ蛋白表达增高，NF-κB蛋白表达降低（P均<0.05）；SHR＋QDH组比SHR＋QDL组效果更显著（P<0.05）。结论   QD可能通过激活心肌PPARγ，从而阻遏心肌肥厚相关蛋白NF-κB的表达，对改善和逆转高血压心室肥厚起着有益的作用。

关键词: 芩丹胶囊；心室肥厚；自发性高血压大鼠；过氧化物酶增殖物激活受体γ；核因子-κB

Abstract:

Objective   To observe the effects of Qindan Capsule (QD) on inhibition of ventricular hypertrophy and the expression of NF-κB in spontaneously hypertensive rats (SHR) by way of activating PPARγ so as to investigate the mechanism of QD reversing hypertension ventricular hypertrophy. Methods   Spontaneously hypertensive rats（SHR）were divided into the model group（SHR group）, QD high dosage group（SHR+QDH group）, QD low dosage group（SHR+QDL group）, and SHR+Telmisartan group（SHR+Tel group）. Wistar-Kyoto group（WKY）rats of the same weeks were taken as the normal control（WKY group）. All rats were administered with corresponding medicine or normal saline. Animal blood pressures were measured by tail-cuff plethysmography.Morphological changes of myocardial tissue were evaluated by HE staining. Ultramicrostructural changes of myocyte were determined by transmission electron microscopy. Expressions of PPARγ and NF-KB were detected by immunohistochemical staining. Results   Compared with those of the SHR group, the systolic blood pressures and the myocardial expressions of NF-κB in the SHR+QDH group, SHR+QDL group and SHR+Tel group were significantly reduced by QD or Tel (P<0.05). In the contrast, the myocardial expressions of PPARγ in the SHR+QDH group, SHR+QDL group and SHR+ Tel group increased by QD or Tel compared to those of the SHR group (P<0.05). The extent of ventricular hypertrophy in the SHR+QDH group was drasticly less than that in the SHR+QDL group(P<0.05). Conclusion   QD ameliorates the ventricular hypertrophy by enhancing the PPARγ expression so as to suppressing the NF-KB expression in myocyte.

Key words: Qindan capsule;  Ventricular hypertrophy;   Spontaneously hypertensive rats；Peroxisome proliferater-activated receptor γ； Nuclear factor kappa-B

中图分类号: 

  • R544.1
[1] 葛汝青1，张继东1，黄山英2，王博1，任敏1，冯利1，郭雪峰1,吕怡静1. 芩丹胶囊对高血压血管外膜重构及TGF-β1/Smad信号转导通路的影响[J]. 山东大学学报(医学版), 2011, 49(4): 7-12.
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