Omega-3多不饱和脂肪酸对角化细胞及
成纤维细胞的作用

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (9): 50-54.

Omega-3多不饱和脂肪酸对角化细胞及
成纤维细胞的作用

郝薇1，李福海2，王玉林1

1.山东大学附属省立医院小儿科，济南 250021； 2.山东大学齐鲁医院小儿科，济南 250012

收稿日期:2012-05-02 出版日期:2012-09-10 发布日期:2012-09-10
通讯作者: 王玉林(1955- )，男，主任医师，主要从事儿童营养学及转化医学研究。 E-mail:Wangyulin07@sina.com
作者简介:郝薇(1979- )，女，博士研究生，主要从事儿童营养学研究

Effects of omega-3 polyunsaturated fatty acids on
keratinocytes and fibroblasts

HAO Wei1, LI Fu-hai2, WANG Yu-lin1

1. Department of Pediatrics, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China；
2. Department of Pediatrics, Qilu Hospital of Shandong University, Jinan 250012, China

Received:2012-05-02 Online:2012-09-10 Published:2012-09-10

摘要/Abstract

摘要：

目的观察Omega3多不饱和脂肪酸对人表皮角化细胞增殖和人皮肤成纤维细胞分化的作用。方法体外培养人表皮角化细胞和人皮肤成纤维细胞，用不同浓度Omega-3多不饱和脂肪酸(EPA)干预角化细胞，BrdU法测角化细胞增殖；不同浓度EPA干预成纤维细胞，ELISA法检测ɑ平滑肌肌动蛋白(ɑ-SMA)及人胶原蛋白1的表达；MTT法观察EPA对成纤维细胞活性的影响。结果 EPA浓度在200μmol/L时，对人表皮角化细胞有明显的促增殖作用，显著提高成纤维细胞培养液中ɑSMA的表达，降低人胶原蛋白1的表达。结论 EPA可能通过促进角化细胞增殖、诱导成纤维细胞分化改善伤口愈合。

关键词: Omega-3多不饱和脂肪酸；角化细胞；成纤维细胞；伤口愈合；增殖；分化

Abstract:

Objective To observe effects of omega-3 polyunsaturated fatty acids (PUFAs) on keratinocyte proliferation and fibroblast differentiation. Methods Keratinocytes and fibroblasts were cultured in vitro. The cells were treated with omega-3 PUFAs at different concentrations. BrdU assay was used to examine the keratinocyte proliferation. The cell toxicity of eicosapentaenoic acid (EPA) was assessed by MTT. ELISA assay was used to observe the expressions of ɑ-SMA and human collagen-1. Results At a concentration of 200μmol/L, EPA significantly promoted keratinocyte proliferation, increased the expression of ɑ-SMA and decreased the expression of human collagen-1 in fibroblasts. Conclusion EPA could increase keratinocyte proliferation and promote fibroblast differentiation, which might promote wound healing.

Key words: Omega-3 polyunsaturated fatty acids; Keratinocytes; Fibroblasts; Wound healing; Proliferation; Differentiation

中图分类号:

R151.2

郝薇1，李福海2，王玉林1. Omega-3多不饱和脂肪酸对角化细胞及
成纤维细胞的作用[J]. 山东大学学报(医学版), 2012, 50(9): 50-54.

HAO Wei1, LI Fu-hai2, WANG Yu-lin1. Effects of omega-3 polyunsaturated fatty acids on
keratinocytes and fibroblasts[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(9): 50-54.

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