酒精性脂肪肝形成过程中肝细胞脂肪性病变的机制

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (7): 19-25.

酒精性脂肪肝形成过程中肝细胞脂肪性病变的机制

王冬梅1，王兵2，于聪1，吴琦3，李霞1，王向东1

1.山东大学医学院细胞生物学研究所，济南 250012； 2.潍坊高新区生物医药科技园，山东潍坊 261000；
3.山东大学医学院人体解剖与组织胚胎学研究所，济南250012

收稿日期:2012-02-11 出版日期:2012-07-10 发布日期:2012-07-10
通讯作者: 李霞(1963- )，女，副教授，主要从事细胞生物学研究。 E-mail：lixia1666@sdu.edu.cn
作者简介:王冬梅(1986- )女，硕士研究生，主要从事细胞生物学研究。 E-mail：dongmei245@163.com

Mechanism of hepatic steatosis in alcoholic fatty liver disease formation

WANG Dong-mei1, WANG Bing2, YU Cong1, WU Qi3, LI Xia1, WANG Xiang-dong1

1. Institute of Cell Biology, School of Medicine，Shandong University， Jinan 250012， China;
2. Science and Technology Park of Biomedicine, Gaoxin District, Weifang 261000， Shandong， China;
3. Institute of Human Anatomy and Histology Embryology, School of Medicine， Shandong University, Jinan 250012， China

Received:2012-02-11 Online:2012-07-10 Published:2012-07-10

摘要/Abstract

摘要：

目的研究乙醇引起肝细胞脂肪性病变的机制。方法以5d龄KM乳小鼠肝细胞为实验材料，分为未分离纯化与分离纯化两大组原代培养，每大组均设正常对照组和乙醇实验组(50、100、200、300、400mmol/L 5个乙醇浓度)，采用形态学观察，油红O染色检测肝细胞脂肪变性的程度，RTPCR检测PPARα与PPARγmRNA水平，以及TNF-α、IL6mRNA水平的变化，综合评价乙醇引起细胞脂肪性病变的机制。结果未分离纯化的肝细胞发生了向脂肪细胞转变的现象；其PPARγ、TNFα和IL6的mRNA水平较其正常对照组有明显提高。结论纯化的肝细胞不易形成脂细胞；而乙醇处理肝脏细胞时，可能是肝细胞在非实质细胞及其产生的因子(包括免疫因子)的作用下发生了肝细胞脂肪变性即去分化或转分化的现象。

关键词: 脂肪肝，酒精性；肝细胞；脂肪变性；转分化；机制

Abstract:

Objective To study the mechanism of hepatic steatosis induced by ethanol. Methods Primarily cultured hepatocytes obtained from KM mouse at the 5th day were divided into two groups: the primarily cultured liver cells and the purified hepatocytes. Each group was divided into the control group and the experiment groups(50, 100, 200, 300 and 400mmol/L of ethanol).The hepatic steatosis degrees were evaluated by morphological changes and the oil red Ostaining. The changes of PPARα, PPARγ, TNF-α and IL-6 at mRNA level were tested with RT-PCR technique. Results The primarily cultured liver cells underwent hepatic steatosis after the ethanol treatment, and the expressions of PPARγ, TNF-α and IL-6 at mRNA level improved obviously. Conclusion Purified hepatocytes lose the ability to trans differentiate into fat cells. It is possible that the transdiferentiation of hepatocytes into fat cells is due to the ability of non-hepatocytes to provide some factors including immune factors upon ethanol stimulation.

Key words: Fatty liver， alcoholic; Hepatocytes； Steatosis; Transdiferentiation； Merchanism

中图分类号:

Q952.6

王冬梅1，王兵2，于聪1，吴琦3，李霞1，王向东1. 酒精性脂肪肝形成过程中肝细胞脂肪性病变的机制[J]. 山东大学学报(医学版), 2012, 50(7): 19-25.

WANG Dong-mei1, WANG Bing2, YU Cong1, WU Qi3, LI Xia1, WANG Xiang-dong1. Mechanism of hepatic steatosis in alcoholic fatty liver disease formation[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(7): 19-25.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed