Smac小分子compound 3调节顺铂诱导卵巢癌细胞SKOV3凋亡的研究

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (6): 61-.

Smac小分子compound 3调节顺铂诱导卵巢癌细胞SKOV3凋亡的研究

乔真1，刘培淑2，韩冰1，王毓1

山东大学 1.医学院，济南 250012； 2.齐鲁医院妇产科，济南 250012

收稿日期:2011-11-16 出版日期:2012-06-10 发布日期:2012-06-10
通讯作者: 刘培淑(1963- )，教授，博士生导师，主要从事妇科肿瘤临床及基础研究。 E-mail:peishuliu@126.com
作者简介:乔真(1985- )，女，硕士研究生，从事妇科肿瘤基础研究
基金资助:
国家自然科学基金面上项目(30872739)

Smac mimic potentiates cisplatin-induced cell apoptosis of
ovarian cancer cells

QIAO Zhen1, LIU Pei-shu2, HAN Bing1, WANG Yu1

1. The medical school of Shandong University, Jinan 250012， China；
2. Department of obstetrics and gynecology of Qilu Hospital, Jinan 250012， China

Received:2011-11-16 Online:2012-06-10 Published:2012-06-10

摘要/Abstract

摘要：

目的探讨Smac小分子compound 3调节顺铂对卵巢癌细胞SKOV3凋亡及生长抑制作用。方法噻唑蓝法检测Smac小分子compound 3单独应用组、顺铂单独应用组及二者联合应用组对卵巢癌细胞SKOV3的生长抑制率、存活率的影响；并绘制生长曲线。Annexin V/PI双标流式细胞术检测Smac小分子compound 3单独应用组、顺铂单独应用组及二者联合应用组对SKOV3凋亡率的影响。Western印迹检测Smac小分子compound 3单独应用组、顺铂单独应用组及二者联合应用组对SKOV3的X连锁凋亡抑制蛋白(X-linked inhibitor of apoptosis protein,XIAP) 、半胱氨酸-天冬氨酸蛋白酶-3(caspase-3)的表达影响。结果 Smac小分子compound 3单独应用组、顺铂单独应用组及二者联合应用组12、24、48h SKOV3生长受到抑制，且呈时间依赖性。二者联合应用组与顺铂单独应用组比较生长抑制率明显升高。二者联合应用组的凋亡率高于顺铂单独应用组及Smac小分子compound 3单独应用组。Smac小分子compound 3单独应用组及二者联合应用组SKOV3的XIAP表达降低，二者联合应用组的caspase3的激活片段表达明显高于其他两组。结论 Smac小分子compound 3能够调节顺铂对SKOV3生长抑制作用，促进顺铂对卵巢癌细胞SKOV3凋亡作用。Smac小分子compound 3通过影响XIAP的表达来实现其促凋亡及生长抑制作用。

关键词: 卵巢肿瘤；Smac小分子 compound 3；SKOV3细胞；细胞凋亡；顺铂

Abstract:

Objective To investigate the effect of a small Smac mimic molecule(compound 3) on cisplatin-induced cell apoptosis and growth inhibition of ovarian cancer cell lines. Methods Cell viability and the growth inhibition ratio of the ovarian cancer cell lines SKOV3 were measured by MTT in the compound 3-treated group, the cisplatin-treated group and the combination treatment group. Apoptosis and protein expression were detected by flow cytometry and Western blot. Results Cell viability was inhibited in the three groups, and it was time-dependent. The growth inhibition ratio of SKOV3 in the combination treatment group was more significantly increased than the cisplatintreated group, and the apoptotic rate of SKOV3 in the combination treatment group was more significantly increased than in the other groups. Expressions of XIAP in SKOV3 cells were decreased in the compound 3-treated group and the combination treatment group. Activated fragments of caspase 3 in the combination treatment group were more increased than in the other groups. Conclusion Treatment with compound 3 enhances growth inhibition and apoptosis of SKOV3 induced by cisplatin. The proapoptotic effect of compound 3 was correlated with expression of XIAP.

Key words: Ovarian cancer; Smac mimic molecule; SKOV3 cell; Apoptosis; Cisplatin

中图分类号:

R711.75

乔真1，刘培淑2，韩冰1，王毓1. Smac小分子compound 3调节顺铂诱导卵巢癌细胞SKOV3凋亡的研究[J]. 山东大学学报(医学版), 2012, 50(6): 61-.

QIAO Zhen1, LIU Pei-shu2, HAN Bing1, WANG Yu1. Smac mimic potentiates cisplatin-induced cell apoptosis of
ovarian cancer cells[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(6): 61-.

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