cyclinD1在卵巢癌裸鼠移植瘤组织中的表达及意义

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (6): 57-60.

cyclinD1在卵巢癌裸鼠移植瘤组织中的表达及意义

代军1，刘培淑1，马道新2，常新忠1，冯进波1，尹格平3

1．山东大学齐鲁医院妇产科，济南 250012； 2.山东大学齐鲁医院肿瘤中心，济南 250012；
3.济南军区总医院妇产科，济南 250010

收稿日期:2012-01-05 出版日期:2012-06-10 发布日期:2012-06-10
通讯作者: 刘培淑(1963- )，女，教授，博士生导师，主要从事妇科肿瘤的基础及临床研究。 E-mail：peishuliu@126.com
作者简介:代军(1971- )，女，硕士研究生，副主任医师，主要从事妇科肿瘤研究
基金资助:
山东省自然科学基金资助课题(Y2001C03)

Expressions of cyclinD1 in ovarian cancer transplanted into nude mice

DAI Jun1, LIU Pei-shu1， MA Dao-xin2, CHANG Xin-zhong1, FENG Jin-bo1, YIN Ge-ping3

1. Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan 250012;
2. Cancer Center, Qilu Hospital of Shandong University, Jinan 250012;
3. Department of Obstetrics and Gynecology, Jinan Military General Hospital, Jinan 250010, China

Received:2012-01-05 Online:2012-06-10 Published:2012-06-10

摘要/Abstract

摘要：

目的探讨细胞周期蛋白D1(cyclinD1)在上皮性卵巢癌裸鼠移植瘤组织中的表达及意义。方法构建人上皮性卵巢癌3AO裸鼠皮下移植瘤模型；设阴性对照组及早、中、晚期3个肿瘤组，应用免疫组化方法及流式细胞术(FCM)检测正常组织及移植瘤卵巢癌组织中cyclinD1的表达。结果 3AO卵巢癌裸鼠皮下移植瘤模型平均成瘤率91.7%。正常组织cyclinD1表达阴性或弱阳性，而卵巢癌组织中cyclinD1呈过表达(54.63%)。早、中、晚期卵巢癌组织中cyclinD1阳性表达率分别是35.00%、55.60%和73.30%，肿瘤细胞凋亡率分别是7.97%、3.45%及1.83%，Mean值分别是23.15、37.31、63.28。结论 cyclinD1在卵巢癌组织中呈过表达,且其表达与移植瘤生长增殖呈正相关,cyclinD1的表达与卵巢癌发生发展关系密切。

关键词: 上皮性卵巢肿瘤；细胞周期素D1；小鼠，裸；免疫组织化学；流式细胞术

Abstract:

Objective To investigate the significance of cyclinD1 expression in ovarian cancer transplanted into nude mice. Methods Human 3AO epithelial ovarian cancer subcutaneously transplanted models in nude mice were established. Expressions of cyclinD1 in normal tissues and ovarian cancer tissues were detected by immunochemistry and flow cytometry. Results Transplanted tumor models of ovarian cancer were successfully established in nude mice , and the mean success rate was 91.7%. CyclinD1 expression was negative in normal tissues. Overexpressions of cyclinD1 were detected in ovarian cancer tissues， and the positive rates were 35.00%, 55.60% and 73.30%. The apoptotic rates were 7.97%, 3.45% and 1.83%. Mean numbers of cyclinD1 were 23.15, 37.31 and 63.28. Conclusion CyclinD1 up-regulation can be detected in ovarian cancer tissues. There is a positive correlation between the proliferation of tumor cells and expression of cyclinD1. CyclinD1 expression is related to the ovarian carcinoma development.

Key words: Epithelial ovarian neoplasms； CyclinD1； Mice, nude； Immunochemistry; Flow Cytometry

中图分类号:

R737.3

代军1，刘培淑1，马道新2，常新忠1，冯进波1，尹格平3. cyclinD1在卵巢癌裸鼠移植瘤组织中的表达及意义[J]. 山东大学学报(医学版), 2012, 50(6): 57-60.

DAI Jun1, LIU Pei-shu1， MA Dao-xin2, CHANG Xin-zhong1, FENG Jin-bo1, YIN Ge-ping3. Expressions of cyclinD1 in ovarian cancer transplanted into nude mice[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(6): 57-60.

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