Toll样受体2、4及核因子-κB在溃疡性结肠炎黏膜组织中的表达及临床意义

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (2): 60-.

Toll样受体2、4及核因子-κB在溃疡性结肠炎黏膜组织中的表达及临床意义

刘胜楠，卢雪峰，孙娜，郑雪婷，李倩倩

山东大学齐鲁医院消化内科，济南 250012

收稿日期:2011-10-13 出版日期:2012-02-10 发布日期:2012-02-10
通讯作者: 卢雪峰(1963- )，男，教授，主要从事消化道肿瘤的早期诊断与治疗研究。 E-mail: luxuefeng@sdu.edu.cn
作者简介:刘胜楠(1985- )，女，硕士研究生，主要从事炎症性肠病的基础与临床研究。

Toll-like receptors 2 and 4 and nuclear factor-ΚB in colonic mucosa of
ulcerative colitis: expression and clinical significance

LIU Sheng-nan, LU Xue-feng, SUN Na, ZHENG Xue-ting, LI Qian-qian

Department of Digestive Medicine, Qilu Hospital of Shandong University, Jinan, Shan 250012, China

Received:2011-10-13 Online:2012-02-10 Published:2012-02-10

摘要/Abstract

摘要：

目的探讨Toll样受体2、4(TLR2、TLR4)与核因子-κB(NF-κB)在溃疡性结肠炎(UC)黏膜组织中的表达，以及三者在UC发病机制中的作用。方法采用免疫组化法检测70例UC患者肠黏膜组织TLR2、TLR4与NF-κB的表达水平，并与44例结肠息肉患者的正常黏膜组织对比。结果 TLR2、TLR4和NF-κB在UC组表达率均高于对照组(P值均＜0.01)；且随内镜分级增高，UC组TLR2、TLR4和NF-κB的表达阳性细胞数均呈逐渐增加趋势(P值均＜0.05)，三者在活动期和非活动期中的表达也存在统计学差异(P值均＜0.01),NF-κB分别与TLR2、TLR4呈显著正相关(r=0.823, P＜0.01； r=0.752, P＜0.01)。结论 UC组织中TLR2、TLR4和NF-κB表达异常，可能在UC发病机制中起重要作用，检测其变化对疾病活动度的评价具有重要的临床意义。

关键词: 溃疡性结肠炎；Toll样受体2；Toll样受体4；核因子-κB

Abstract:

Objective To investigate expression of Toll-like receptors 2 and 4(TLR2、TLR4) and nuclear factor-κB(NF-κB) in colonic mucosa of ulcerative colitis. Methods Immunohistochemistry was performed to examine the expression of TLR2、TLR4 and NF-κB in colonic mucosa of UC (n=70). Normal mucosa of colon polyps was included as a control (n=44). Results The expression of all three proteins in colonic mucosa of UC was higher than that in control (P＜0.01). Cell numbers with positive expression were parallel to the endoscopic classification grades in UC (P values＜0.05). Expression of the three proteins in active UC was significantly higher than in unactive UC (P values＜0.01). Further, the expression of NF-κB was positively correlated with TLR2 and TLR4(r=0.823, P＜0.01；r=0.752, P＜0.01, respectively). Conclusion The elevated expression of TLR2、TLR4 and NF-κB in UC may play a role in UC pathogenesis and, hence, may be of clinical significance.

Key words: Ulcerative colitis; Toll-like receptor 2; Tolllike receptor 4; nuclear factor-κB

中图分类号:

R574.1， R392.3

刘胜楠，卢雪峰，孙娜，郑雪婷，李倩倩. Toll样受体2、4及核因子-κB在溃疡性结肠炎黏膜组织中的表达及临床意义[J]. 山东大学学报(医学版), 2012, 50(2): 60-.

LIU Sheng-nan, LU Xue-feng, SUN Na, ZHENG Xue-ting, LI Qian-qian. Toll-like receptors 2 and 4 and nuclear factor-ΚB in colonic mucosa of
ulcerative colitis: expression and clinical significance[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(2): 60-.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed

[1]	陈艳1，李秀梅2，王洪江3，庞作良3，李卉4，姜孝芳4，马莉莉1，谌宏鸣5，李惠武4. 新疆哈萨克族食管癌c-myc的表达及其调控机制[J]. 山东大学学报(医学版), 2011, 49(11): 135-.
[2]	江娜. 奥沙拉嗪联合甘草酸二铵对溃疡性结肠炎患者脂质过氧化损伤和IL-1β水平的影响[J]. 山东大学学报(医学版), 2011, 49(9): 114-116.
[3]	王志强1,2，于振海2，袁方曙1. 双歧杆菌对溃疡性结肠炎大鼠肠黏膜上皮细胞Toll样受体2、4及NF-κB基因表达的影响[J]. 山东大学学报(医学版), 2011, 49(5): 10-14.