藤梨根提取物对肺癌A549细胞裸鼠移植瘤的抑制作用

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (2): 38-42.

藤梨根提取物对肺癌A549细胞裸鼠移植瘤的抑制作用

孙雪飞，裴艳涛，尹秋伟，杨国涛，王德江

山东大学齐鲁医院胸外科，济南 250012

收稿日期:2011-07-01 出版日期:2012-02-10 发布日期:2012-02-10
通讯作者: 杨国涛（1964- ），男，教授，主任医师，博士研究生导师，主要从事胸部肿瘤的基础研究。E-mail：yanggtxwk@163.com
作者简介:孙雪飞（1974- ），男，副教授，副主任医师，硕士研究生导师，主要从事肺癌的基础研究。
基金资助:
山东省中青年科学家科研奖励基金资助项目（2007BS03061）

Inhibitory effect of Radix Actinidiae extractive on transplantation
tumor of the lung cancer cell line A549 in nude mice

SUN Xue-fei, PEI Yan-tao,YIN Qiu-wei, YANG Guo-tao, WANG De-jiang

Department of Thoracic Surgery, Qilu Hospital of Shandong University, Jinan 250012, China

Received:2011-07-01 Online:2012-02-10 Published:2012-02-10

摘要/Abstract

摘要：

目的研究藤梨根乙酸乙酯提取物对肺癌A549细胞裸鼠移植瘤的生长抑制及凋亡诱导作用，并探讨其作用机制。方法建立肺癌A549细胞裸鼠移植瘤模型，观察藤梨根乙酸乙酯提取物的作用，设置对照组、治疗组（低剂量组、高剂量组）。通过测量移植瘤体积变化绘制生长曲线，根据终末瘤质量比较计算抑制率，采用TUNEL法检测移植瘤凋亡指数，采用免疫组化法检测移植瘤Survivin蛋白表达、增殖指数，采用逆转录-聚合酶链式反应（RT-PCR）检测移植瘤Survivin mRNA变化。结果各治疗组经藤梨根乙酸乙酯提取物治疗后移植瘤生长缓慢，治疗结束时治疗组移植瘤质量及瘤体积明显低于对照组，高、低剂量组的瘤质量抑制率分别为69.00%和45.09%。各治疗组移植瘤组织凋亡指数则明显高于对照组，而Survivin mRNA及蛋白表达较对照组明显减少，高剂量组尤其明显，各组间上述指标比较差异均有统计学意义（P<0.01）。结论藤梨根乙酸乙酯提取物在体内对肺癌A549细胞生长有抑制作用，可以诱导移植瘤细胞发生凋亡，促进癌细胞凋亡并下调瘤细胞Survivin 蛋白和mRNA表达。

关键词: 藤梨根提取物；肺肿瘤；小鼠，裸；细胞凋亡；基因，Survivin；免疫组织化学；逆转录聚合酶链式反应

Abstract:

Objective To study the inhibitory effect and apoptosis-inducing action of acetidin extractive of Radix Actinidiae on transplantation tumor of lung cancer A549 cells in nude mice, and to explore their mechanisms. Methods A model of transplantation tumor bearing lung cancer A549 cells was established in nude mice, and the effect of acetidin extractive of Radix Actinidiae in vivo was detected. The control group and treatment groups (highdose group and low-dose group)were set for comparison．According to the transplantation tumor volume, the transplantation tumor growth curve was drawn. According to the final tumor weight, the inhibitory ratio was calculated. Terminal deoxynucleotidyl transferase mediated UTP nick end labeling (TUNEL) was used to determine the apoptosis index of transplantation tumor. Expressions of Survivin protein and mRNA in transplantation tumor were detected by immunohistochemistry (SP) and RT-PCR, respectively. Results　The growth of transplantation tumor in treatment groups was slow after taking drug. The final tumor volume and tumor weight in treatment groups were smaller than those in the control group. Tumor weight inhibitory rates in the high-dose group and low-dose group were 69.00% and 45.09%, respectively. The apoptosis index of transplantation tumor in treatment groups was higher than that in the control group. Expression of Survivin mRNA and protein in treatment groups were lower than those in the control group, and they was more signifi-cantly decreased in the high-dose group. Differences were significant in the above indexes between each two groups(P<0.01). Conclusion The acetidin extractive of Radix Actinidiae has an inhibitory effect on growth of transplantation tumor of lung cancer A549 cells in nude mice, inhibiting proliferation activity of transplantation tumor, degrading expressions of Survivin protein and mRNA, and inducing apoptosis of cancer cells.

Key words: Radix Actinidiae extractive; Lung neoplasms; Mice, nude; Apoptosis; Gene, Survivin; Immunohistochemistry; Reverse transcriptase polymerase chain reaction

中图分类号:

R734.2

孙雪飞，裴艳涛，尹秋伟，杨国涛，王德江. 藤梨根提取物对肺癌A549细胞裸鼠移植瘤的抑制作用[J]. 山东大学学报(医学版), 2012, 50(2): 38-42.

SUN Xue-fei, PEI Yan-tao,YIN Qiu-wei, YANG Guo-tao, WANG De-jiang. Inhibitory effect of Radix Actinidiae extractive on transplantation
tumor of the lung cancer cell line A549 in nude mice[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(2): 38-42.

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