关键词: 蛋白质组学; 人类; 血浆; 声门区喉癌

Abstract:

Objective   Different serum protein levels in pre- and post-operative T1 glottic laryngeal carcinoma patients were analyzed using proteomics methods to determine the tumor biomarker proteins. Methods   Comparative proteomic analysis of human sera between pre-and post-operative T1 glottic laryngeal carcinoma patients was performed using two-dimensional (2D) gel electrophoresis, in combination with matrix-assisted laser desorption ionization time of flight mass spectrometry and database searching, to determine alternative proteins in T1 glottic laryngeal carcinoma patients. Western blot analysis, as well as the measurement of malondialdehyde (MDA) and superoxide dismutase (SOD) levels, was also performed. Results   Image analysis of 2D gels indicated that 16 protein spots corresponding to 12 proteins exhibited significant different expression levels between the two groups of T1 glottic laryngeal carcinoma patients. Six protein expression levels were upregulated. Western blot analysis confirmed the results of proteome analysis. MDA levels in the pre- treatment group were higher than those in the control and post-treatment groups. By contrast, SOD levels in the pretreatment group were lower than those in the control and post-treatment groups. Conclusion   Proteomics results combined with bioinformatics analysis indicate the important roles of oxidant stress and immune response as targets in the monitoring, as well as treatment, of T1 glottic laryngeal carcinoma. The differentially expressed proteins may also reflect the pathological progress of T1 glottic laryngeal carcinoma and should be studied further.

Key words: Proteomic analysis; Human; Serum; Glottic laryngeal carcinoma