类固醇合成酶在亚临床库欣综合征患者中的表达及意义

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (12): 87-.

类固醇合成酶在亚临床库欣综合征患者中的表达及意义

杨学成1，孙瑞霞2，范靓靓3，王开平2，矫文娟2，
曹彩霞2，高燕燕2，孙立江1，王新生1

青岛大学医学院附属医院 1.泌尿外科；2.内分泌科；3.心电诊断科，山东青岛 266003

收稿日期:2012-07-31 出版日期:2012-12-10 发布日期:2012-12-10
通讯作者: 曹彩霞(1975- )，女，博士，主治医师，主要从事内分泌性高血压和代谢综合征的研究与治疗。E-mail: yuheangel@yahoo.com.cn
作者简介:杨学成(1980- )，男，博士，主治医师，主要从事肾上腺肿瘤的研究与治疗。E-mail:yangxuecheng80@yahoo.com.cn
基金资助:
北京市科委2011年度科技计划重大项目（SCW201106）；山东省科技攻关课题（2011YD18006）。

Expression of steroidogenic enzyme in subclinical Cushing’s
syndrome due to adrenal adenomas

YANG Xue-cheng1, SUN Rui-xia2, FAN Liang-liang3, WANG Kai-ping2, JIAO Wen-juan2,
CAO Cai-xia2, GAO Yan-yan2, SUN Li-jiang1, WANG Xin-sheng1

1. Department of Urology；2.Department of Endocrinology；3. Department of ECG Diagnosis,
the Affiliated Hospital of Medical College，Qingdao University， Qingdao 266003， China

Received:2012-07-31 Online:2012-12-10 Published:2012-12-10

摘要/Abstract

摘要：

目的通过检测类固醇合成酶CYP11B1和CYP17在亚临床库欣综合征腺瘤(SCS)、正常肾上腺皮质(NA)、肾上腺无功能瘤(NFA)及皮质醇分泌瘤(CPA)中的表达，探讨SCS的分泌状态是否与类固醇合成酶的表达相关。方法提取20例CPA、6例SCS、15例NFA、8例NA的组织RNA，采用实时荧光定量PCR、Western印迹及免疫组织化学方法检测CYP17、CYP11B1mRNA及蛋白在不同肾上腺组织中的表达，比较不同组织中二者表达的差异，并与患者的临床资料行相关性分析。结果 CYP17、CYP11B1mRNA及蛋白在不同组织中均有表达。二者在CPA中高表达，在SCS中表达稍高于NA和NFA (P<0.05)。二者在CPA中的表达与血清皮质醇水平呈正相关，与ACTH水平呈负相关(P<0.05)。结论亚临床库欣综合征腺瘤中高表达的CYP11B1和CYP17可能与患者皮质醇分泌异常相关。

关键词: 肾上腺皮质腺瘤；皮质醇；类固醇合成酶；亚临床库欣综合征

Abstract:

Objective To determine whether steroidogenic enzyme expression is associated with the steroid secretory pattern of subclinical Cushing’s syndrome (SCS) by investigating the expression of CYP17 and CYP11B1 in SCS adenomas, normal adrenal cortices (NA), non-functional adrenal adenomas (NFA) and cortisol-producing adenomas (CPA). Methods Total RNA and protein were extracted from 20 CPA, 6 SCS, 15 NFA, and 8 NA. Real-time quantitative polymerase chain reaction (PCR), western blotting and immunohistochemistry analysis were performed to determine the mRNA and protein expression of CYP17 and CYP11B1 in different tissues. Expressions of CYP17 and CYP11B1 in the adrenocortical tumors were compared in NA. Results Expressions of both CYP11B1 and CYP17 mRNA and protein were detected in all samples tested. Expressions of CYP11B1 mRNA and protein were significantly higher in CPA group than the others, and were slightly higher in SCS samples than that in NA and NFA samples (P<0.05). Expressions of CYP17 mRNA and protein in different tissues were similar to that of CYP11B1. They showed positive correlations with plasma cortisol and negative correlations with adrenocorticotrophin levels (P<0.05). Conclusion CYP11B1 and CYP17 were over-expressed in subclinical CPA,and their over-expressions were caused by the increased production of cortisol in CPA.

Key words: Adrenocortical tumor； Cortisol； Steroidogenic enzyme； Subclinical Cushing’s syndrome

中图分类号:

R589

杨学成1，孙瑞霞2，范靓靓3，王开平2，矫文娟2，曹彩霞2，高燕燕2，孙立江1，王新生1. 类固醇合成酶在亚临床库欣综合征患者中的表达及意义[J]. 山东大学学报(医学版), 2012, 50(12): 87-.

YANG Xue-cheng1, SUN Rui-xia2, FAN Liang-liang3, WANG Kai-ping2, JIAO Wen-juan2, CAO Cai-xia2, GAO Yan-yan2, SUN Li-jiang1, WANG Xin-sheng1. Expression of steroidogenic enzyme in subclinical Cushing’s
syndrome due to adrenal adenomas[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(12): 87-.

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