关键词: 热休克蛋白90；Ki-67；胃肿瘤；组织芯片；预后

Abstract:

Objective   To explore the expression of heat shock protein 90β (HSP90β) in gastric adenocarcinoma and its correlation with proliferation and clinicopathological parameters of the tumor cells, and to analyze the relationship of HSP90β with the patients′ prognosis. Methods   Use immunohistochemistry PV-9000 two-step method to detect the expression of HSP90β and Ki-67 in 60 cases of gastric adenocarcinoma and 20 cases of gastritis,  the χ2 test was used to analyze the relationship between clinicopathological parameters,  the Φ coefficient was used to discuss the correlation between the HSP90β and Ki-67,  and survival analysis was performed on 60 patients with gastric cancer. Results   Expressions of HSP90β and Ki-67 in gastric adenocarcinoma were higher than those in gastritis tissues (P<0.05),  and the positive rate in poorly differentiated gastric adenocarcinoma was much higher than that in well and moderately differentiated gastric adenocarcinoma (P<0.05);there was a positive correlation of expression between HSP90β and Ki-67 in gastric adenocarcinoma(Φ=0.368,  P<0.05).The survival period of patients with HSP90β positive expression was less than that of patients with negative expression. Conclusion   HSP90β is significantly up-regulated in gastric carcinoma. Its expression is positively correlated to Ki-67 and heralded a poor prognosis.

Key words: Heat shock protein 90；Ki-67；Gastric neoplasms；Tissue microarray; Prognosis