牛蒡低聚果糖对脂多糖诱导下RAW264.7细胞炎症模型的抗炎作用

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (12): 41-.

牛蒡低聚果糖对脂多糖诱导下RAW264.7细胞炎症模型的抗炎作用

刘晶1，潘晓华2，宋珍1，程梅华3，王墨林1

1. 山东大学医学院医学遗传学研究所，济南 250012； 2. 山东大学附属省立医院普通外科，济南 250021；
3.济宁市任城区计划生育服务站，山东济宁 212100

收稿日期:2012-06-27 出版日期:2012-12-10 发布日期:2012-12-25
通讯作者: 王墨林（1974- ），女，博士，副教授，硕士生导师，主要从事遗传病的相关研究。E-mail：wml@sdu.edu.cn
作者简介:刘晶（1986- ），女，硕士研究生，主要从事天然药物保护作用的相关研究。 E-mail：lzp90715@163.com
基金资助:
国家自然科学基金青年项目（30500190）;国家自然科学基金主任基金(31240031);山东省自然科学基金（ZR2012HQ016）；山东省优秀中青年科学家奖励基金（2008BS03024）

Anti-inflammatory effect of burdock fructo-oligsaccharide on
lipopolysaccharide-stimulated RAW264.7 cell

LIU Jing1， PAN Xiao-hua2, SONG Zhen1， CHENG Mei-hua3， WANG Molin1

1. Institute of Medical Genetics, School of Medicine, Shandong University, Jinan 250012, China；
2. Department of General Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250012, China；
3. Family Planning Service Station of Jining City, Jining 212100, Shandong, China

Received:2012-06-27 Online:2012-12-10 Published:2012-12-25

摘要/Abstract

摘要：

目的观察牛蒡低聚果糖（BFOS）对RAW264.7炎症细胞模型的抗炎作用。方法用脂多糖（LPS）处理巨噬细胞系RAW264.7细胞，建立炎症模型，并用不同浓度BFOS处理。采用定量PCR检测环氧合酶2（COX-2）、一氧化氮合成酶（iNOS）、单核细胞趋化蛋白1（MCP-1）、白细胞介素6（IL-1）等炎性因子的表达水平，ELISA检测BFOS处理前后MCP-1、IL-6的分泌量。Western blot法检测COX-2、iNOS、κB抑制因子（IκB）、核转录因子κB p65（NF-κBp65）、细胞外信号调节激酶（Erk）、P38、c-Jun氨基末端激酶（JNK）的磷酸化蛋白表达水平。结果与对照组相比，经BFOS处理后，细胞iNOS、COX-2、MCP-1及IL-6的水平表达显著下降(P<0.01)。BFOS处理组p-IκB、p-NF-κBp65、p-Erk、p-P38、p-JNK等蛋白表达较LPS单独处理组显著下降(P<0.01)。结论 BFOS对LPS 诱导的RAW264.7细胞炎症模型具有抗炎作用。

关键词: 牛蒡低聚果糖；抗炎作用；RAW264.7细胞系；核转录因子κB；有丝分裂原活化蛋白激酶

Abstract:

Objective To investigate the anti-inflammatory effect of burdock fructo-oligsaccharide (BFOS) on the inflammatory model of RAW264.7 cells. Methods The inflammation model was constructed by treating RAW264.7 cells with lipopolysaccharide (LPS), and treated with different doses of BFOS. Expressions of Cyclooxygenase (COX-2), Nitric oxide synthases (iNOS), monocyte chemotactic protein-1 (MCP-1) and Interleukin-6(IL-6) were detected by realtime PCR. Expressions of MCP-1 and IL-6 were determined by ELISA before and after the treatment with BFOS. Expressions of COX-2, iNOS, phosphorylated IκB, NF-κBp65, extracellular signal-regulated (Erk), P38 and c-Jun Nterminal kinase (JNK) were detected by Western blot. Results The expression levels of iNOS，COX-2，MCP-1 and IL-6 of RAW264.7 cells stimulated by LPS increased, and BFOS significantly decreased them . BFOS decreased the expressions of p-IκB，p-NF-κBp65，p-Erk，p-P38，and p-JNK compared to the control. Conclusion BFOS may exert anti-inflammatory effect on RAW264.7 cells stimulated by LPS.

Key words: Burdock fructo- oligsaccharide; Anti-inflammatory effect; RAW264.7 cell line; Nuclear factor-κappa B； Mitogen-activated protein kinase

中图分类号:

R394.6

刘晶1，潘晓华2，宋珍1，程梅华3，王墨林1. 牛蒡低聚果糖对脂多糖诱导下RAW264.7细胞炎症模型的抗炎作用[J]. 山东大学学报(医学版), 2012, 50(12): 41-.

LIU Jing1， PAN Xiao-hua2, SONG Zhen1， CHENG Mei-hua3， WANG Molin1. Anti-inflammatory effect of burdock fructo-oligsaccharide on
lipopolysaccharide-stimulated RAW264.7 cell[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(12): 41-.

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