头痛宁对偏头痛大鼠c-fos和c-jun基因表达的影响

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (11): 62-.

头痛宁对偏头痛大鼠c-fos和c-jun基因表达的影响

董玲芬1，邢毅2，孙灵芝3，李旭阳1，张潇丹1，李淑玲1

1.山东大学齐鲁医院中医科，济南 250012； 2.山东大学附属省立医院神经外科，济南 250021；
3.山东中医药大学附属医院神经内科，济南 250012

收稿日期:2012-05-11 出版日期:2012-11-10 发布日期:2012-11-10
通讯作者: 李淑玲(1949- )，女，教授，主任医师，博士生导师，主要从事中西医结合妇科、内分泌以及神经病学的研究。 E-mail: qilu_lishuling@163.com
作者简介:董玲芬(1985- )，女，硕士研究生，主要从事中西医结合神经病学、妇科以及内分泌疾病的研究。

Effects of Toutongning on expressions of c-fos and
c-jun in the rat migrainous model

DONG Ling-fen1, XING Yi2, SUN Ling-zhi3, LI Xu-yang1, ZHANG Xiao-dan1, LI Shu-ling1

1. Department of Traditional Chinese Medicine, Qilu Hospital of Shandong University, Jinan 250012, China;
2. Department of Neurosurgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021,China;
3. Department of Neurology, Affiliated Hospital of Shandong Traditional Chinese Medicine University, Jinan 250012， China

Received:2012-05-11 Online:2012-11-10 Published:2012-11-10

摘要/Abstract

摘要：

目的探讨头痛宁对偏头痛大鼠c-fos和c-jun基因表达的影响。方法 Wistar大鼠60只，分为生理盐水组、偏头痛模型组、盐酸氟桂利嗪组，头痛宁高、中、低剂量组，除生理盐水组外，其余各组注射硝酸甘油建立偏头痛模型，并用免疫组化方法观察cfos 和cjun基因的表达。结果偏头痛模型组cfos和cjun阳性细胞数增多，与生理盐水组相比有统计学差异(P<0.01)；头痛宁高剂量组和盐酸氟桂利嗪组c-fos和c-jun阳性细胞数减少，与偏头痛模型组比较有统计学差异(P<0.01)；头痛宁中剂量组c-fos和c-jun阳性细胞数减少，与偏头痛模型组比较有统计学差异(P<0.05)；头痛宁低剂量组c-fos阳性细胞数减少，但与偏头痛模型组比较无统计学差异；头痛宁低剂量组c-jun阳性细胞数减少，与偏头痛模型组有统计学差异(P<0.05)。结论头痛宁可以减弱由硝酸甘油诱导的致痛物质c-fos、c-jun的表达，从而抑制痛觉信息的传递。

关键词: 镇痛药；偏头痛；基因表达；大鼠，Wistar

Abstract:

Objective To explore the effect of Toutongning on the expression of c-fos and c-jun in the migrainous model. Methods 60 Wistar rats, in both genders, were randomly divided into 6 groups: the normal saline(NS)group, the model group, the Flunarizine Hydrochloride group, the high dose of Toutongning group, mid dose group, and low dose group. The nitroglycerin migrainous animal model was constructed with the nitroglycerin (NTG)subcutaneous injection. The expression of c-fos and c-jun was detected with the immunohistochemical method. Results c-fos and c-jun positive cells in the model group significantly increased compared with the NS group(P<0.01). The expression of c-fos and c-jun in the high-dose Toutongning group and Flunarizine Hydrochloride group decreased compared with the model group(P<0.01). The expression of c-fos and c-jun in the mid-dose Toutongning group decreased compared with the model group(P<0.05). The expression of c-fos in the low-dose Toutongning group decreased, but there was no significant difference between the model group and the low-dose Toutongning group. The c-jun positive cell number in the low-dose Toutongning group decreased, compared with the model group(P<0.05). Conclusion Toutongning can lower the expression of c-fos and c-jun induced by nitroglycerin, and can inhibit the transmission of nociceptive pain information.

Key words: Analgesics; Migraine; Gene expression; Rats, Wistar

中图分类号:

R259

董玲芬1，邢毅2，孙灵芝3，李旭阳1，张潇丹1，李淑玲1. 头痛宁对偏头痛大鼠c-fos和c-jun基因表达的影响[J]. 山东大学学报(医学版), 2012, 50(11): 62-.

DONG Ling-fen1, XING Yi2, SUN Ling-zhi3, LI Xu-yang1, ZHANG Xiao-dan1, LI Shu-ling1. Effects of Toutongning on expressions of c-fos and
c-jun in the rat migrainous model[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(11): 62-.

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