整合素-β1在转化生长因子-β2诱导晶状体上皮细胞转化中的作用

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (11): 58-.

整合素-β1在转化生长因子-β2诱导晶状体上皮细胞转化中的作用

朱玉广,朱艳,王杰,钟莹莹,杜孝楠,张荣

潍坊医学院附属医院眼科中心，山东潍坊 261031

收稿日期:2012-03-19 出版日期:2012-11-10 发布日期:2012-11-10
通讯作者: 朱艳(1975- )，女，讲师，主要从事青光眼和白内障的研究。 E-mail:azhu1975@sina.com
作者简介:朱玉广(1972- )，男，博士，副教授，主要从事青光眼和白内障的研究。
基金资助:
山东省中青年科学家科研奖励基金(BS2009SW016);山东省卫生厅科技计划(2009HZ105)；山东省自然科学基金(Y2008C127)；山东省教育厅科技计划(J08LG13)。

Integrin β1 is involved in trans-differentiation of len epithelial
cells induced by transforming growth factor-β2 in vitro

ZHU Yu-guang, ZHU Yan, WANG Jie, ZHONG Ying-ying, DU Xiao-nan, ZHANG Rong

Eye Center, Affiliated Hospital of Weifang Medical College, Weifang 261031, Shandong, China

Received:2012-03-19 Online:2012-11-10 Published:2012-11-10

摘要/Abstract

摘要：

目的探讨整合素-β1(integrin β1)在转化生长因子-β2 ( TGF-β2 )体外诱导晶状体上皮细胞(HLECs)向肌成纤维细胞转化中的作用。方法体外培养的HLECs，选择传3代的细胞进行实验，以TGF-β2作为诱导剂。免疫荧光方法检测HLECs integrin β1、E-cadherin、α-SMA和F-actin的表达，RT-PCR方法检测E-cadherin 和α-SMA mRNA的表达。其中部分细胞预先加入去整合素kistrin孵育1h，再加入TGF-β2刺激细胞48h。结果 TGF-β2处理晶状体上皮细胞后，integrin β1、α-SMA和F-actin的表达明显增强，细胞E-cadherin表达明显减弱。加入去整合素kistrin后，细胞α-SMA和F-actin的表达明显减弱，细胞E-cadherin表达明显增强。结论 TGF-β2诱导了HLECs转分化，整合素-β1参与了TGF-β2诱导的HLECs向肌成纤维细胞的转化过程。

关键词: 整合素-β1;转化生长因子-β2;晶状体上皮细胞; 转分化;去整合素

Abstract:

Objective To investigate the effect of integrin β1 in trans-differentiation of human len epithelial cells(HLECs) induced by transforming growth factor-β2 (TGF-β2) in vitro. Methods HLECs were cultured and passaged． The 3rd generation of HLECs were treated with TGF-β2． Expressions of integrin β1, E-cadherin,α-SMA and F-actin in the cells were analyzed with immunofluorescent staining. Expressions of α-SMA mRNA and Ecadherin mRNA were detected by RTPCR. For some cells, disintegrin kistrin was pre-treated 1h followed by TGF-β2 incubation in 48h. Results Expressions of integrin β1,α-SMA and F-actin were significantly increased after treatment of TGF-β2, while expression of E-cadherin was significantly decreased after treatment of TGF-β2． Disintegrin kistrin partly reversed TGF-β2 induced expressions o f a-SMA, F-actin and Ecadherin. Conclusion TGF-β2 induced trans-differentiation of HLECs towards myofibroblasts. Integrin β1 was involved in initiation of the trans-differentiation induced by TGF-β2.

Key words: Integrin β1; Transforming growth factor; Human len epithelial cells; Transdifferentiation; Kistrin

中图分类号:

R776

朱玉广,朱艳,王杰,钟莹莹,杜孝楠,张荣. 整合素-β1在转化生长因子-β2诱导晶状体上皮细胞转化中的作用[J]. 山东大学学报(医学版), 2012, 50(11): 58-.

ZHU Yu-guang, ZHU Yan, WANG Jie, ZHONG Ying-ying, DU Xiao-nan, ZHANG Rong. Integrin β1 is involved in trans-differentiation of len epithelial
cells induced by transforming growth factor-β2 in vitro[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(11): 58-.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed