关键词: 信号转导子和转录激活子3；RNA干扰；胃癌；增殖

Abstract:

Objective   To construct siRNA expression vectors targeting the signal transducer and activator of transcription 3(STAT3) gene and to examine the inhibitory effect of the siRNAs on the expression of STAT3 and its effect on proliferation of stomach cancer cell line HGC-27. Methods   Two STAT3-siRNA fragments were synthesized, and then transfected into HGC-27 cells. STAT3 mRNA and protein were examined by RT-PCR and Western blot, respectively. Inhibition on proliferation was determined by the MTT assay. ResultsThe two STAT3-siRNA expression vectors were found to contain the correct siRNA sequences, as confirmed by sequencing and enzymatic digestion. Results    of RT-PCR and Western blot showed that the abundances of STAT3 mRNA and protein were significantly decreased relative to both the control and si-NC groups (P<0.01), results with STAT3-siRNA1 being more pronounced than with STAT3-siRNA2. MTT assay showed that, after transfection, cell proliferation in the siRNA group was reduced significantly compared with the si-NC group (P<0.01). Conclusion   Expression of STAT3-siRNA in stomach cell line HGC-27 can effectively inhibit STAT3 expression, leading to proliferation suppression.

Key words: Signal transducer and activator of transcription 3； RNA interference； Stomach cancer； Proliferation