关键词: 缺血预调置；缺血/再灌注损伤；心肌缺血；一氧化氮

Abstract:

Objective   To discuss the relation of NO with ischemic myocardia preconditioning function.  Methods   To take 300～350g male health Wistar rats into control, I/R, preconditioning, L-NNA and L-Arg＋L-NNA four groups, respectively to observe the vary of CPP and myocardial protein leaks out, LDH, cGMP as well as MDA contents of myocardial injured index index.  Results   As compared with I/R group, Myocardial protein leaks out quantity obviously reduces, LDH leaks out quantity obviously decreases, and myocardial MDA content obviously reduces, cGMP level obviously elevates, All have a statistical significance(P<0.01). CPP reduces (P<0.05). L-NNA perfusion group may interdict myocardial protection of preconditioning, LNNA+L-Arg perfusion group can reverse myocardial protection of preconditioning interdicted by L-NNA perfusion group. Conclusion    Ischemic preconditioning obviously enhances the ischemic myocardia tolerance to ischemia and anoxia, it is closely related to improve NO deficiency in myocardial I/R injure.

Key words: Ischemic preconditioning； Ischemia/reperfusion injury； Myocardial ischemia； Nitric Oxide