关键词: 神经管缺损；高温，诱发；cDNA文库；基因，CDK109；金仓鼠

Abstract:

Objective   To screen for gene CDK109 from the cDNA library of the neural tube from golden hamster treated with hyperthermia, clone this gene and analyze its relativity with neural tube defects（NTDs） induced by hyperthermia. Methods   With BentonDavis hybridization, a oligonucleotide probe was used to screen the cDNA library of the neural tube from golden hamster model treated with hyperthermia, which had been constructed in our laboratory. Then the positive phage clone was transfected into E.coli BM25.8, the plasmid was isolated and identified by restriction enzyme digestion, and the positive plasmid was sequenced and blasted. By Northern blot, expression of screened gene CDK109 was detected and reconfirmed in heat-treated and control groups of golden hamsters on pregnant day 8.5. Results   The whole length of the CDK109 gene was successfully screened from the cDNA library of the neural tube from golden hamsters treated with hyperthermia. Its expression in the heat-treated group was evidently higher than in the control group.  Conclusion   Increased expression of CDK109 is closely related to neural tube defects induced by hyperthermia.

Key words: Neural tube defects； Hyperthermia, induced； cDNA library； Genes, CDK109； Mesocricetus