环氧合酶-2介导促炎因子脂多糖诱导的脊髓长时程增强的变化

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (1): 15-18.

环氧合酶-2介导促炎因子脂多糖诱导的脊髓长时程增强的变化

杨红卫1,王翔宇2

1.三峡大学医学院生理学教研室, 湖北宜昌 443002;
2.葛洲坝中心医院神经内科三峡大学第三临床学院, 湖北宜昌 443000

收稿日期:2011-08-24 出版日期:2012-01-10 发布日期:2012-01-10
作者简介:杨红卫(1968- )，男，副教授, 主要从事病理性疼痛的中枢和外周机制及脂类活性物质在突触传递与可塑性中的作用机制研究。
基金资助:
国家自然科学基金资助面上项目(30970930)，宜昌市科学技术研究与开发项目(A0930124)，三峡大学人才科研启动基金资助项目(KJ2009B010)

COX-2 mediates changes of LPS-induced spinal long-term potentiation

YANG Hong-wei1, WANG Xiang-yu2

1. Department of Physiology, College of Medical Science, China Three Gorges University，
Yichang 443002, Hubei, China; 2. Department of Neurology, Gezhouba Central Hospital,
Third Clinical Hospital of China Three Gorges University， Yichang 443000, Hubei, China

Received:2011-08-24 Online:2012-01-10 Published:2012-01-10

摘要/Abstract

摘要：

目的探讨环氧合酶2(COX2)和促炎因子脂多糖(LPS)对脊髓长时程增强(LTP)的作用。方法采用细胞外记录技术,在脊髓腰膨大部记录背角浅层神经元C-纤维诱发电位，蛋白质印迹检测LPS处理后不同时间点COX-2蛋白的表达。结果腹腔注射 LPS后4h脊髓LTP显著增强，COX-2蛋白的表达显著增高；LPS注射后12、24h对脊髓LTP无明显作用，COX-2的表达无明显增强；COX-2的选择性抑制剂NS398可翻转LPS注射4h后对脊髓背角LTP的增强效应，NS398可显著抑制LPS 4h后对脊髓COX-2表达的增强。结论 COX-2的激活参与LPS诱导的脊髓LTP的变化。

关键词: 环氧合酶-2；脂多糖类；长时程增强；脊髓

Abstract:

Objective To explore the roles of cycclooxygenase-2 (COX-2) and proinflammatory cytokine lipopolysaccharide (LPS) in spinal long-term potentiation (LTP). Methods The C-fiber-evoked field potentials were recorded at superficial layers of the spinal dorsal horn at the lumbar enlargement, and the COX-2 expression in LPS-treated rats at different time points was measured by immunoblotting technique. Results Spinal LTP and COX-2 expression were significantly augmented 4h, but not 12h and 24h after intraperitoneal injection of LPS. NS398, a selective inhibitor of COX-2, reversed the elevated LTP induced by LPS 4h after injection. Elevated COX-2 expression in LPS-treated rats 4h after injection was significantly suppressed by NS398. Conclusion Activation of COX-2 is involved in changes of LPS-induced spinal LTP.

Key words: Cycclooxygenase-2; Lipopolysaccharides; Long-term potentiation; Spinal cord

中图分类号:

R338.2

杨红卫1,王翔宇2. 环氧合酶-2介导促炎因子脂多糖诱导的脊髓长时程增强的变化[J]. 山东大学学报(医学版), 2012, 50(1): 15-18.

YANG Hong-wei1, WANG Xiang-yu2. COX-2 mediates changes of LPS-induced spinal long-term potentiation[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(1): 15-18.

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