关键词: 流感病毒；感染模型；小鼠；实时荧光RT-PCR

Abstract:

Objective   To establish a mouse infection model of human seasonal influenza virus(H1N1), and make preparations for the study on influenza viral pathogenicity and new antiviral drugs.  Methods     Mice were inoculated with influenza virus(H1N1) by the nasal cavity. After 4 days, mice with serious infectious signs were killed. Pneumonic homogenate that was confirmed with influenza virus infection by real time fluorescence quantitative polymerase chain  reaction (FQ-PCR) was injected into chick embryo allantoic cavity. Allantoic fluid was inoculated in the next generation of mice. When infected mice presented obvious infectious signs, the 50% lethal dose (LD50) was calculated. The virus of 10LD50 was inoculated into mice to establish the infection model. Then, general condition, weight, lung index, and pathological sections were observed and viral RNA was detected by FQ-PCR. Results   After 4 times of passage, the mouse infection model of human seasonal influenza virus (H1N1) was established, with LD50 of 10-2.41/0.05mL. The mouse infection model had serious infectious signs, poor general condition, decreased weight, increased lung index, positive results by FQ-PCR and mortality of 70%. Conclusion   The mouse infection model of human seasonal influenza virus (H1N1) has been successfully established.

Key words: Influenza virus; Infection model; Mice；Real-time fluorescence quantitative polymerase chain reaction