白癜风患者外周血CD4+CD25+ Foxp3+调节性T细胞的检测及意义

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (1): 133-.

白癜风患者外周血CD4+CD25+ Foxp3+调节性T细胞的检测及意义

李强1,2，蔡大幸1，陈光3，刘继峰4，李春阳1

1.山东大学齐鲁医院皮肤科, 济南 250012；2.济宁医学院附属金乡医院皮肤科山东金乡 272200；
3.中国医科大学附属一院皮肤科, 沈阳 110001； 4.杭州市第三人民医院皮肤科, 杭州 310009

收稿日期:2011-07-21 出版日期:2012-01-10 发布日期:2012-01-10
通讯作者: 李春阳，教授，博士生导师，主要从事真菌病的防治与致病真菌的研究。 Email: lichunyangql@sdu.edu.cn
作者简介:李强(1976- )，博士研究生，副主任医师，主要从事色素障碍性皮肤病与机体免疫关系的研究。

Detection and significance of CD4+CD25+ Foxp3+ regulatory T cells in peripheral blood of patients with vitiligo

LI Qiang1,2, CAI Da-xing1, CHEN Guang3, LIU Ji-feng4, LI Chun-yang1

1. Department of Dermatology, Qilu Hospital of Shandong University, Jinan 250012, China;
2. Department of Dermatology, Jinxiang Hospital Affiliated to Jining Medical College, Jinxiang 272200, Shandong, China;
3. Department of Dermatology, First Hospital Affiliated to China Medical University, Shenyang 110001, China;
4. Department of Dermatology, Third People′s Hospital of Hangzhou City, Hangzhou 310009, China

Received:2011-07-21 Online:2012-01-10 Published:2012-01-10

摘要/Abstract

摘要：

目的检测白癜风患者外周血CD4+CD25+ Foxp3+调节性T细胞水平变化及免疫调节剂对其表达的影响，进一步明确其在白癜风免疫治疗中的意义。方法白癜风患者36例，进展期24例，稳定期12例。采用流式细胞术检测不同病期白癜风患者外周血CD4+CD25+ Foxp3+调节性T细胞水平，并与20例正常人进行对照。对进展期白癜风患者给予免疫调节剂等进行综合治疗。结果进展期患者外周血中CD4+CD25+ Foxp3+调节性T细胞数量低于正常对照组(P<0.05)；稳定期患者与正常对照组比较差异无统计学意义(P＞0.05)；经卡介菌多糖核酸治疗后，进展期患者CD4+CD25+ Foxp3+调节性T细胞数量高于治疗前(P<0.05)，略低于正常对照组(P＞0.05)，稳定期患者与治疗前比较差异无统计学意义(P＞0.05)。结论白癜风患者外周血中CD4+CD25+ Foxp3+调节性T细胞的数量存在异常，使体内免疫功能处于失衡状态，卡介菌多糖核酸可能通过调节CD4+CD25+ Foxp3+调节性T细胞的表达水平，调节白癜风患者的细胞免疫功能，从而发挥治疗作用。

关键词: 白癜风；CD4+CD25+Foxp3+调节性T细胞；卡介菌多糖核酸；免疫调节

Abstract:

Objectives To detect CD4+CD25+Foxp3+ regulatory T cells in peripheral blood of patients with vitiligo, and to evaluate immunomodulator′s therapeutic effects. Methods 36 patients with vitiligo were selected, including 24 with active vitiligo and 12 with stationary vitiligo. The level of CD4+CD25+Foxp3+ regulatory T cells in peripheral blood was detected by flow cytometry before and after treatment with immunomodulator, 20 healthy subjects as controls. Results CD4+CD25+Foxp3+ regulatory T cells in active vitiligo were significantly lower than in normal controls(P<0.05). There was no statistical difference between stationary vitiligo and normal controls(P＞0.05). CD4+CD25+Foxp3+ regulatory T cells in active vitiligo increased after comprehensive treatment with immunomodulator(P<0.05); while there was no statistical difference in stationary vitiligo after treatment with immunomodulator(P＞0.05). Conclusion CD4+CD25+Foxp3+ regulatory T cells could control the autoimmune reaction by inhibiting autoreactive T cells. The abnormal number of CD4+CD25+ Foxp3+ regulatory T cells in peripheral blood may cause imbalance of immunity. Polysaccharide nucleic acid fraction of bacillus calmette guerin(BCGPSN) could modulate the cell immune function by regulating the level of CD4+CD25+ Foxp3+ regulatory T cells and plays therapeutic effects.

Key words: Vitiligo; CD4+CD25+Foxp3+regulatory T cells; Polysaccharide nucleic acid fraction of bacillus calmette guerin; Immunoregulation

中图分类号:

R758.41

李强1,2，蔡大幸1，陈光3，刘继峰4，李春阳1. 白癜风患者外周血CD4+CD25+ Foxp3+调节性T细胞的检测及意义[J]. 山东大学学报(医学版), 2012, 50(1): 133-.

LI Qiang1,2, CAI Da-xing1, CHEN Guang3, LIU Ji-feng4, LI Chun-yang1. Detection and significance of CD4+CD25+ Foxp3+ regulatory T cells in peripheral blood of patients with vitiligo[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(1): 133-.

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