肺结核患者杀伤细胞免疫球蛋白样受体基因多态性分析

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (1): 122-.

肺结核患者杀伤细胞免疫球蛋白样受体基因多态性分析

路超1，沈亚娟1，邓云峰2，王长印1，张炳昌1，范刚1，赵跃然3，
刘义庆1，赵胜梅1，王子娥1，张春芝1，卢志明1

1.山东大学附属省立医院检验科，济南 250021； 2.山东省胸科医院感染办公室，济南 250013；
3.山东大学附属省立医院科学研究中心，济南 250021

收稿日期:2011-06-07 出版日期:2012-01-10 发布日期:2012-01-10
通讯作者: 卢志明(1965- )，男，博士生导师，主要从事分子免疫学的研究。 Email: luzhiming@sdu.edu.cn
作者简介:路超(1985- )，女，博士研究生，主要从事分子免疫学的研究。
基金资助:
山东省科学技术攻关基金资助项目(2009GG10002014)

Polymorphisms of killer cell immunoglobulin-like receptor genes in patients with pulmonary tuberculosis

LU Chao1, SHEN Ya-juan1, DENG Yun-feng2, WANG Chang-yin1, ZHANG Bing-chang1, FAN Gang1, ZHAO Yue-ran3, LIU Yi-qing1, ZHAO Sheng-mei1, WANG Zi-e1, ZHANG Chun-zhi1, LU Zhi-ming1

1. Department of Laboratory Medicine, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China;
2. Infection Office, Shandong Chest Hospital, Jinan 250013, China;
3. Center for Science Research, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China

Received:2011-06-07 Online:2012-01-10 Published:2012-01-10

摘要/Abstract

摘要：

目的探讨杀伤细胞免疫球蛋白样受体(KIRs) 基因多态性与肺结核(TB) 的关系。方法采用序列特异性引物聚合酶链反应(SSPPCR)，分析109例肺结核患者(TB组)和30例健康者(对照组)中14个KIR基因多态性。TB组又分为30例痰涂片抗酸杆菌阳性(SP组)和79例涂片阴性患者(SN组)。结果与对照组比较， SP组 KIR2DL5(P=0.007)、2DS1(P=0.038)、2DS3(P=0.010)、3DS1(P=0.002) 阳性率增高，KIR2DS5频率降低(P=0.038)； SN组KIR2DL5(P=0.018)、2DS1(P=0.000)、2DS5(P=0.000)、3DS1(P=0.001)阳性率增高；KIR2DS4(P=0.004)和KIR2DS5(P=0.000) 频率SP组较SN组升高；患者携带两个及以上活化KIR的频率较对照组明显升高(P=0.013)；患者组基因型AJ的频率较对照组显著降低。结论 KIR基因多态性可能与肺结核的易感性有关。

关键词: 杀伤细胞免疫球蛋白样受体；结核，肺；多态性，单核苷酸

Abstract:

Objective To investigate association of polymorphisms of killer cell immunoglobulin-like receptor(KIR) genes with susceptibility to tuberculosis (TB) infection. Methods Sequence specific primer polymerase chain reaction (SSPPCR) method was used to analyze 14 KIR genes in genomic DNA in 30 smear-positive (SP) patients, 79 smear-negative (SN) patients and 30 healthy controls. Results Frequencies of KIR2DL5 (P=0.007), 2DS1 (P=0.038), 2DS3 (P=0.010) and 3DS1(P=0.002) were statistically significantly higher in the SP group than in the control group, while KIR2DS5(P=0.038) was lower. Also, frequencies of KIR2DL5 (P=0.018), 2DS1 (P=0.000), 2DS5 (P=0.000) and 3DS1 (P=0.001) were significantly higher in the SN group than in the control group. Compared with the SN group, frequencies of KIR2DS4 (P=0.004) and KIR2DS5 (P=0.000) were higher in the SP group. In addition, the number of subjects carrying more than two activating KIR genes in TB group was higher than that in the control group (P=0.013). The frequency of genotype AJ was lower in TB patients than in the controls. Conclusion Polymorphisms of KIR genes may be associated with susceptibility to TB infection.

Key words: Killer cell immunoglobulin-like receptor; Tuberculosis, Pulmonary; Polymorphism, single nucleotide

中图分类号:

R521

路超1，沈亚娟1，邓云峰2，王长印1，张炳昌1，范刚1，赵跃然3，刘义庆1，赵胜梅1，王子娥1，张春芝1，卢志明1 . 肺结核患者杀伤细胞免疫球蛋白样受体基因多态性分析[J]. 山东大学学报(医学版), 2012, 50(1): 122-.

LU Chao1, SHEN Ya-juan1, DENG Yun-feng2, WANG Chang-yin1, ZHANG Bing-chang1, FAN Gang1, ZHAO Yue-ran3, LIU Yi-qing1, ZHAO Sheng-mei1, . Polymorphisms of killer cell immunoglobulin-like receptor genes in patients with pulmonary tuberculosis[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(1): 122-.

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