肝硬化大鼠全肝血流阻断不同时间肺损伤的改变

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (9): 56-.

肝硬化大鼠全肝血流阻断不同时间肺损伤的改变

张永明1，孟珂伟2，黄庆先2，高辉3，董梅4，周先亭2，李绍军2，谢广顺5，汤义军6

1.滨州医学院研究生处，山东烟台 264003； 2.烟台市护士学校临床教研室，山东烟台264000；
3.烟台毓璜顶医院肝胆外科，山东烟台 264000； 4.烟台毓璜顶医院妇产科，山东烟台 264000；
5.烟台毓璜顶医院呼吸内科，山东烟台 264000； 6.烟台毓璜顶医院胸外科，山东烟台 264000

收稿日期:2010-12-20 出版日期:2011-09-10 发布日期:2011-09-10
通讯作者: 孟珂伟(1969- )，男，医学博士，副主任医师，主要从事肝胆胰外科、肝移植研究。E-mail：doctormkw@126.com
作者简介:张永明(1983- )，男，硕士研究生，主要从事肝胆胰外科、肝移植研究。
基金资助:
烟台市科技发展计划课题(200915513)

Changes of lung injury after total hepatic blood flow occlusion in cirrhotic rats at different times

ZHANG Yong-ming1， MENG Ke-wei2， HUANG Qing-xian2， GAO Hui3， DONG Mei4，
ZHOU Xian-ting2， LI Shao-jun2， XIE Guang-shun5， TANG Yi-jun6

1. Department of Graduates, Binzhou Medical University, Yantai 264003, Shandong, China;
2. Department of Clinical Nursing, Yantai Nursing School, Yantai 264000, Shandong, China;
3. Department of Hepatobiliary Surgery, Yantai Yuhuangding Hospital, Yantai 264000, Shandong, China;
4. Department of Obstetrics and Gynecology, Yantai Yuhuangding Hospital, Yantai 264000, Shandong, China;
5. Department of Respiratory, Yantai Yuhuangding Hospital Yantai 264000, Shandong, China;
6. Department of Thoracic Surgery, Yantai Yuhuangding Hospital, Yantai 264000, Shandong, China

Received:2010-12-20 Online:2011-09-10 Published:2011-09-10

摘要/Abstract

摘要：

目的研究肝硬化大鼠全肝血流阻断不同时间后肺组织损伤情况。方法 SD大鼠口服乙醇和CCl4制作肝硬化模型80只，随机分为4组，每组20只。假手术组(A1组)作为对照，采用阻断肝门、肝上上腔静脉及肝下下腔静脉15min(A2组)、30min(A3组)、45min(A4组)制作全肝血流阻断模型，取右肺下叶肺组织常规HE染色病理检查，ELISA法检测肺匀浆TNFα 含量，左肺部分组织作湿/干比值(W/D)测定。结果 ①常规病理切片肺损伤评分：A4组评分最高，A2、A3组次之，A1组最低(P<0.05)。②ELISA法检测肺组织内TNF-α含量，A4组最高，A2、A3组次之，A1组最低(P<0.05)。③W/D：A4组最高，A2、A3组次之，A1组最低(P<0.001)。结论肝硬化大鼠全肝血流阻断后可以造成肺组织损伤，阻断时间超过30min后明显加重。

关键词: 肝硬化；全肝血流阻断；肺；大鼠

Abstract:

Objective To observe lung injury after total hepatic vascular occlusion in cirrhotic rats at different times. Methods A total of 80 SD rats developed liver cirrhosis by receiving alcohol and CCl4, and they were randomly divided into 4 groups(n= 20). The hepatic artery, portal vein, liver superior vena cava and hepatic inferior vena cava were blocked for 15min (group A2), 30min (group A3) and 45min (group A4) to establish a rat model of total hepatic vascular occlusion, rats free from occlusion (group A1) as controls. The lung tissue in the right lung lower lobe was taken for pathological examination by HE staining. The TNF-α level in lung homogenates was measured by ELISA. The wet/dry ratio was determined in part of the left lung lobe. Results The conventional pathological lung injury score ranked in a decreasing order from groups A4, A3, A2 to group A1(P<0.05), and the TNF-α level in the lung measured by ELISA and wet / dry weight ratio of the lung showed the same order (P<0.05 and P<0.001 respectively). Conclusion Lung injury can be caused after total hepatic blood flow occlusion in rats with liver cirrhosis, and the injury is significantly increased after occlusion for more than 30min.

Key words: Cirrhosis; Total hepatic blood flow occlusion; Lung; Rats

中图分类号:

R657.3

张永明1，孟珂伟2，黄庆先2，高辉3，董梅4，周先亭2，李绍军2，谢广顺5，汤义军6. 肝硬化大鼠全肝血流阻断不同时间肺损伤的改变[J]. 山东大学学报(医学版), 2011, 49(9): 56-.

ZHANG Yongming1， MENG Kewei2， HUANG Qingxian2， GAO Hui3， DONG Mei4，ZHOU Xian-ting2， LI Shao-jun2， XIE Guang-shun5， TANG Yi-jun6. Changes of lung injury after total hepatic blood flow occlusion in cirrhotic rats at different times[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(9): 56-.

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