脱细胞真皮基质的制备与性能分析

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (9): 41-44.

脱细胞真皮基质的制备与性能分析

王灵平1，张春莲2，胡顺鹏1，李文波1,3，曹成波1

1.山东大学化学与化工学院，济南 250061； 2.山东大学校医院，济南 250061；
3.青岛大学化学化工与环境学院，山东青岛 266021

收稿日期:2011-01-19 出版日期:2011-09-10 发布日期:2011-09-10
通讯作者: 曹成波(1965- ),男,教授,博士生导师,主要从事生物医用材料、有机合成化学及清洁生产技术等的研究。 E-mail: cbcao@sdu.edu.cn
作者简介:王灵平(1985- ),男,硕士研究生,主要从事生物医用材料的研究。
基金资助:
山东省重点科技攻关项目(2008GG10002031);中国博士后科学基金资助项目(20070421074, 200801401)

Preparation and characterization of acellular dermal matrix

WANG Ling-ping1, ZHANG Chun-lian2, HU Shun-peng1, LI Wen-bo1,3, CAO Cheng-bo1

1. School of Chemistry and Chemical Engineering, Shandong University, Jinan 250061, China;
2. School Hospital, Shandong University, Jinan 250061, China;
3.School of Chemistry and Chemical Engineering and Environment, Qingdao University, Qingdao 266021, Shandong, China

Received:2011-01-19 Online:2011-09-10 Published:2011-09-10

摘要/Abstract

摘要：

目的制备一种新型脱细胞真皮基质(ADM)并分析其性能。方法选用猪皮为原料，采用化学、物理及生物综合技术制备ADM，采用HE染色、扫描电子显微镜观察、红外光谱分析、氨基酸分析、拉力实验、MTT法检测其外观、结构及理化性能。结果 ADM细胞去除彻底，胶原蛋白的含量达92.01%；具有连通的三维孔道，孔径约100μm；红外吸收符合胶原蛋白的吸收特征，保持了胶原纤维的天然结构；透水汽性达到每天2923g/m2；孔隙率提高到85.54%；细胞在ADM上黏附生长、增殖良好、分布均匀。结论 ADM孔隙率和拉伸强度适宜、孔结构理想、降解速度可控、生物相容性良好，符合皮肤组织工程对支架材料的要求。

关键词: 脱细胞真皮基质；孔结构；透水汽性；细胞相容性

Abstract:

Objective To prepare a new type of acellular dermal matrix (ADM) and analyze its properties. Methods A new type of ADM was prepared by means of combined treatments with physical, chemical and biological methods, and its structure, composition and properties were evaluated. Results No cell was observed in ADM, and the content of collagen was about 92.01%. The obtained ADM framework had interconnected pores of about 100μm in diameter, and the natural framework of the collagen kept well. Its water vapor permeability was about 2923g/m2 per day, and the porosity reached 85.54%. MTT assay indicated that cells cultured on ADM adhered to the surface, proliferated well and were evenly distributed. Conclusion The prepared ADM has good microstructure, high mechanical properties and porosity, controlled enzymatic stability and good cell compatibility, and it meets the requirement of tissue engineering for stents.

Key words: Acellular dermal matrix; Microstructure; Water vapor permeability; Cytocompatibility

中图分类号:

R622.1

王灵平1，张春莲2，胡顺鹏1，李文波1,3，曹成波1. 脱细胞真皮基质的制备与性能分析[J]. 山东大学学报(医学版), 2011, 49(9): 41-44.

WANG Ling-ping1, ZHANG Chun-lian2, HU Shun-peng1, LI Wen-bo1,3, CAO Cheng-bo1. Preparation and characterization of acellular dermal matrix[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(9): 41-44.

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