娠期抑郁症对大鼠中缝核色氨酸羟化酶2表达的影响

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (9): 37-40.

娠期抑郁症对大鼠中缝核色氨酸羟化酶2表达的影响

徐晓明，李秋玲，那全，崔红，刘彩霞

中国医科大学附属盛京医院妇产科，沈阳 110004

收稿日期:2010-12-10 出版日期:2011-09-10 发布日期:2011-09-10
通讯作者: 刘彩霞(1958- )，女，教授，博士生导师，主要从事产前诊断与胎儿治疗、妊娠期心理方面的研究。 E-mail：liucx@sjhospital.org
作者简介:徐晓明(1985- )，女，硕士研究生，主要从事妊娠期心理方面的研究。
基金资助:
辽宁省教育厅高等学校科学研究项目(05L458)

Influence of prenatal depression on TPH2 expression in median raphe nuclei of rats

XU Xiao-ming, LI Qiu-ling, NA Quan, CUI Hong, LIU Cai-xia

Department of Obstetrics and Gynecology, Shengjing Hospital Affiliated to China Medical University, Shenyang 110004, China

Received:2010-12-10 Online:2011-09-10 Published:2011-09-10

摘要/Abstract

摘要：

目的探讨妊娠期抑郁症对大鼠中缝核色氨酸羟化酶2(TPH2)表达的影响。方法选取月龄、体质量相近的Wistar大鼠，合笼受孕后将孕鼠随机分为实验组、对照组。实验组给予18d慢性不可预见性的温和应激(CUMS)制备妊娠期抑郁症大鼠模型，对照组在正常条件下饲养。采用旷场实验和1%蔗糖水偏爱实验对大鼠进行行为学评分，采用免疫组化SP法测定大鼠中缝核TPH2的表达。结果 18d CUMS后，实验组大鼠旷场实验评分、蔗糖水消耗实验结果及中缝核TPH2表达量低于对照组(P均<0.01)。实验组大鼠18d CUMS前后水平运动得分差与TPH2表达量呈负相关(r=-0.71)，垂直运动得分差与TPH2表达量呈负相关(r=-0.74)，中央格停留时间差与TPH2表达量呈正相关(r=0.64)，蔗糖水消耗量差与TPH2表达量呈负相关(r=-0.69)。结论妊娠期抑郁症模型大鼠中缝核TPH2表达量下降可能与妊娠期抑郁症的发生有关。

关键词: 妊娠；抑郁症；色氨酸羟化酶2；中缝核；大鼠，Wistar

Abstract:

Objective To investigate influence of prenatal depression on expression of tryptophan hydroxylase 2 (TPH2) in median raphe nuclei of rats. Methods Adult pregnant Wistar rats, similar in age and body weight, were randomly divided into the experimental group and the control group. The rats of the experimental group were exposed to chronic unexpected mild stress (CUMS) for 18 days to establish an animal model. Behavior scores were assessed by the open-field test and fluid consumption test. Expression of TPH2 in median raphe nuclei was detected by the SP immunohistochemical method. Results After 18 days of CUMS, the behavior score, amount of fluid consumption and expression of TPH2 in median raphe nuclei were significantly lower in the experimental group than those in the control group(all P<0.01). Expression of TPH2 was negatively correlated with the horizontal score(r=-0.71), vertical score(r=-0.74)and sugar water consumption amount(r=-0.69), while positively correlated with stop time in the center(r=0.64). Conclusion Decreased expression of TPH2 in median raphe nuclei of depressive pregnant rats may be related to prenatal depression.

Key words: Pregnant; Depression; Tryptophan hydroxylase 2; Median raphe nuclei; Rats, Wistar

中图分类号:

R714.259

徐晓明，李秋玲，那全，崔红，刘彩霞. 娠期抑郁症对大鼠中缝核色氨酸羟化酶2表达的影响[J]. 山东大学学报(医学版), 2011, 49(9): 37-40.

XU Xiao-ming, LI Qiu-ling, NA Quan, CUI Hong, LIU Cai-xia. Influence of prenatal depression on TPH2 expression in median raphe nuclei of rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(9): 37-40.

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