肉豆蔻提取物对IFN-γ干预的小胶质细胞BV2的调控作用

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (9): 21-.

肉豆蔻提取物对IFN-γ干预的小胶质细胞BV2的调控作用

文现宇，毛翘，崔春爱

延边大学基础医学院解剖学教研部，吉林延吉 133002

收稿日期:2011-05-27 出版日期:2011-09-10 发布日期:2011-09-10
通讯作者: 崔春爱(1970- )，女，博士，副教授，硕士生导师，主要从事神经解剖学研究。 E-mail: cuicha@ybu.edu.cn
作者简介:文现宇(1987- )，男，硕士研究生，主要从事神经解剖学的研究。
基金资助:
吉林省教育厅科研基金资助项目［吉教科合字(2011)第4号］

Effects of nutmeg extraction on microglial BV2 cells treated with IFN-γ

WEN Xian-yu， MAO qiao， CUI Chun-ai

Department of Anatomy, School of Basic Medical Science, Yanbian University, Yanji 133002, Jilin, China

Received:2011-05-27 Online:2011-09-10 Published:2011-09-10

摘要/Abstract

摘要：

目的探讨肉豆蔻提取物对鼠性小胶质BV2细胞的作用机制。方法实验分为对照组、IFN-γ模型组、肉豆蔻提取物复合处理组。采用WST-8试剂盒，观察肉豆蔻提取物对体外培养的鼠性小胶质细胞BV2生存率的影响，采用分子生物学技术，检测IFN-γ所诱导的诱导型一氧化氮合酶(iNOS)和环氧合酶2(COX-2)的表达水平。结果肉豆蔻提取物对鼠性小胶质细胞BV2无毒性，且抑制了IFN-γ所诱导的iNOS和COX-2的表达，与IFN-γ模型组比较差异显著(P＜0.01)。结论肉豆蔻提取物对鼠性小胶质细胞BV2具有很好的抗神经毒性及抗炎作用。

关键词: 肉豆蔻；干扰素Ⅱ型；小胶质细胞

Abstract:

Objective To study the effect of nutmeg ethanol extract on murine microglial BV2 cells. Methods Murine microglial BV2 cells were divided into the control group, the IFN-γ model group, and the combined nutmeg ethanol extract treatment group. The WST-8 kit was used to detect the effect of nutmeg ethanol extract on murine microglial BV2 cell viability. The effects of interferon-γ(IFN-γ) and nutmeg ethanol extract on protein expressions of inducible nitric oxide synthase(iNOS) and cyclooxygenase 2(COX-2) in vitro were measured by Western blot. Results Nutmeg ethanol extract treatment did not affect cell viability as determined by the WST-8 cell counting kit. Western blot revealed that nutmeg ethanol extract could inhibit protein expressions of iNOS and COX-2 induced by IFN-γ. There were significant differences between the combined nutmeg ethanol extract treatment group and the IFN-γmodel group(P＜0.01). Conclusion Nutmeg ethanol extract could exert anti-neurotoxicity and anti-inflammatory effects on murine microglial BV2 cells.

Key words: Myristica fragrans; Interferon typeⅡ; Microglial cells

中图分类号:

R745.4+9

文现宇，毛翘，崔春爱. 肉豆蔻提取物对IFN-γ干预的小胶质细胞BV2的调控作用[J]. 山东大学学报(医学版), 2011, 49(9): 21-.

WEN Xian-yu， MAO qiao， CUI Chun-ai. Effects of nutmeg extraction on microglial BV2 cells treated with IFN-γ[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(9): 21-.

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