Vogt-小柳原田病患者外周血Th17细胞的变化及意义

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (9): 153-.

Vogt-小柳原田病患者外周血Th17细胞的变化及意义

陈璇，王玉

济南市第二人民医院眼科，济南 250001

收稿日期:2011-02-28 出版日期:2011-09-10 发布日期:2011-09-10
通讯作者: 王玉(1957- )，男，主任医师，主要从事玻璃体视网膜疾病的研究
作者简介:陈璇(1976- )，女，博士，主治医师，主要从事玻璃体视网膜疾病的研究。

Alteration of Th17 cells in the peripheral blood of patients with Vogt-koyanagi-Harada disease and its significance

CHEN Xuan, WANG Yu

Department of Ophthalmology, Second People′s Hospital of Jinan, Jinan 250001, China

Received:2011-02-28 Online:2011-09-10 Published:2011-09-10

摘要/Abstract

摘要：

目的探讨不同时期Vogt-小柳原田病(VKH)患者外周血Th17细胞的变化和白介素-17(IL-17)的水平及其意义。方法选择30例VKH患者(17例活动期和13例静止期)及20例健康人(对照组)，抽取外周血，应用荧光定量PCR法检测RORγt的表达；分离外周血单个核细胞(PBMC)，应用细胞内细胞因子染色和流式细胞技术检测Th17细胞的数量，并将其培养后应用ELISA方法检测培养液上清液中IL-17的含量。结果活动期VKH患者外周血Th17细胞的数量和其特异性转录因子RORγt的基因表达水平均明显高于静止期患者和正常对照组。同时活动期VKH患者外周血PBMC分泌的IL-17水平也明显高于静止期VKH患者及健康人，两者比较均差异有统计学意义(P<0.05)。静止期VKH患者Th17细胞的数量和RORγt的基因表达水平及IL-17的含量与正常对照组比较差异无统计学意义(P＞0.05)。结论活动期VKH患者Th17细胞数量增多，且IL-17的水平增高，因此Th17细胞可能在VKH患者葡萄膜炎的发生中发挥重要的作用。

关键词: Vogt-小柳原田病；Th17细胞；IL-17；RORγt

Abstract:

Objective To investigate the alteration of Th17 cells and the level of IL-17 in patients with Vogt-koyanagi-Harada disease (VKH) and their significance. Methods Blood samples were taken from 30 patients with VKH disease(17 with active uveitis and 13 with inactive uveitis) and 20 healthy control subjects. The proportion of Th17 cells in peripheral blood mononuclear cells (PBMCs) was analyzed with flow cytometry. Real-time PCR was performed to evaluate mRNA expression of retinoid-related orphan receptor γt (RORγt). The level of IL-17 was determined by ELISA. Results Expression of RORγt mRNA, the proportion of Th17 cells and the level of IL-17 were significantly higher in VKH patients with active uveitis than in VKH patients with inactive uveitis and normal control subjects(P<0.05). While there was no difference between VKH patients with inactive uveitis and normal control subjects(P＞0.05). Conclusion Th17 cells and IL-17 increase in VKH patients with active uveitis and may be responsible for the development of uveitis in patients with VKH.

Key words: Vogt-koyanagi-Harada disease； Th17 cells； IL-17； Retinoid-related orphan receptor γt

中图分类号:

R773.9

陈璇，王玉. Vogt-小柳原田病患者外周血Th17细胞的变化及意义[J]. 山东大学学报(医学版), 2011, 49(9): 153-.

CHEN Xuan, WANG Yu. Alteration of Th17 cells in the peripheral blood of patients with Vogt-koyanagi-Harada disease and its significance[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(9): 153-.

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