通心络对糖尿病大鼠心肌RAGE、NF-κB的影响

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (8): 16-20.

通心络对糖尿病大鼠心肌RAGE、NF-κB的影响

尹梅1，高海青1，李保应1，程梅1，吴守彩1,王昊2

1.山东大学齐鲁医院老年病科，山东省心血管蛋白质组学重点实验室，济南 250012;
2.山东大学齐鲁医院重症医学科, 济南 250012

收稿日期:2010-12-08 出版日期:2011-08-10 发布日期:2011-08-10
通讯作者: 高海青(1952- )，男，教授，博士生导师，主要从事老年心血管病的基础及临床研究。 E-mail：gaohaiqing52@yahoo.com.cn
作者简介:尹梅(1983- )，女，医师，硕士研究生，主要从事老年病的临床及超声诊治。 E-mail：yinmeiql1220@yahoo.com.cn
基金资助:
山东省中医药科技发展计划项目(2009-154)。

Effects of Tongxinluo on the expressions of RAGE and NF-κB in diabetic rats

YIN Mei1, GAO Haiqing1, LI Baoying1, CHENG Mei1, WU Shoucai1, WANG Hao2

1. Department of Geriatrics, Qilu Hospital of Shandong University, Key Laboratory of Cardiovascular
Proteomics of Shandong Province, Jinan 250012, China;
2. Intensive Care Unit Department, Qilu Hospital of Shandong University, Jinan 250012, China

Received:2010-12-08 Online:2011-08-10 Published:2011-08-10

摘要/Abstract

摘要：

目的研究通心络对糖尿病大鼠心肌组织糖基化终末产物受体(RAGE)及核转录因子(NFκB)表达的影响，探讨其对糖尿病大鼠心肌的保护作用及机制。方法雄性Wistar大鼠60只，随机选出15只作为正常对照组(C组，n=15)，剩余45只尾静脉注射0.1%链脲佐菌素(STZ)建立糖尿病模型，成模大鼠40只，随机分为糖尿病模型组(DM 组，n=20) 和糖尿病通心络治疗组(TXL组，n=20)，通心络500mg／(kg·d)。喂养24周后，各组大鼠存活数为 C组12只、DM组9只、TXL组13只，处死大鼠迅速心脏穿刺取血，测空腹血糖、糖化血红蛋白(HbA1c) 及糖基化终末产物(AGEs)等指标。RT-PCR及Western blot法测定大鼠心肌组织中RAGE和NFκB的表达。结果与C组相比，DM组大鼠血清中AGEs水平及心肌组织中RAGE和NF-κB的表达显著升高(P均<0.05)。经通心络治疗后，TXL组大鼠血清中AGEs的水平与DM组无统计学差异(P>0.05)，但心肌组织中RAGE和NF-κB的表达较DM组显著降低(P均<0.05)。结论通心络可能通过抑制糖尿病大鼠心肌组织RAGE及NF-κB的表达而对糖尿病大鼠心肌组织起到一定的保护作用。

关键词: 通心络；糖基化终末产物；糖基化终末产物受体；核转录因子；大鼠，Wistar

Abstract:

Objective To investigate the effects of Tongxinluo on the expressions of RAGE and NF-κB in diabetic rats. Methods 15 rats were chosen randomly from 60 healthy male Wistar rats as the normal control group(C group, n=15). The other 45 rats were subjected to 0.1% STZ through the tail veins to induce diabetes. Then 40 rats were randomly divided into two groups: the diabetic model group (DM group, n=20) and the Tongxinluo-treatment diabetic group (TXL group, n=20, Tongxinluo 500mg/(kg·d)). After 24 weeks,there were 12 rats in C group,9 ones in DM group and 13 ones in TXL group.All rats were sacrificed, and the fasting plasma glucose, glycosylated hemoglobin and AGEs were determined. Myocardial expressions of RAGE and NF-κB were detected by RT-PCR and Western blot. Results Compared with C group, Plasma AGEs and myocardial expressions of RAGE and NF-κB were higer in DM group(P<0.05). As for AGEs,there was no significant difference between DM group and TXL group(P>0.05), however, the myocardial expressions of RAGE and NF-κB of TXL group were both lower than those of DM group(P<0.05). Conclusion Tongxinluo may exert protective effects on myocardium by suppressing the expressions of RAGE and NF-κB in diabetic rats.

Key words: Tongxinluo; Advanced glycosylation end products; Advanced glycosylation end product receptor; Nuclear factor-kappa B; Rats, Wistar

中图分类号:

R542.2

尹梅1，高海青1，李保应1，程梅1，吴守彩1,王昊2. 通心络对糖尿病大鼠心肌RAGE、NF-κB的影响[J]. 山东大学学报(医学版), 2011, 49(8): 16-20.

YIN Mei1, GAO Haiqing1, LI Baoying1, CHENG Mei1, WU Shoucai1, WANG Hao2. Effects of Tongxinluo on the expressions of RAGE and NF-κB in diabetic rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(8): 16-20.

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