关键词: 盐酸氨基乙酰丙酸；痤疮；模型，动物；光动力疗法；疗效；兔

Abstract:

Objective     To investigate the therapeutic effect of aminolevulinic acid(ALA) linimentum on exprimental acne of New Zealand rabbits′ ears. Methods      Thirty-two healthy adult New Zealand rabbits were used in this experiment. Propionibacterium acnes and oleic acid were administrated to the right inner ears (2cm×2cm range) of the rabbits to establish acne models. Then all the animals were randomly divided into 5 groups， ① the model control group: photodynamics therapy(PDT) was given after solvent was applied in the disease region, once per week for 3 weeks, ② the clindamycin metronidazole acne positive control group: clindamycin metronidazole was smeared in the disease region， 3 times/d for 2 weeks, ③ the retinoid positive control group: retinoid was applied in the disease region, 3 times/d  for 2 weeks， ④ the ALA single treatment group: ALA was applied in the disease region, once in the first week, ⑤ the ALA repeated treatment group: ALA was applied in the disease region, once per week for 3 weeks, and ⑥the normal control group. The animals in the ALA treatment groups and model control group were protected from light for 3 hours after administration, and then ALA photodynamic therapy(ALA-PDT) was given for 15 min/time. Aftertreatment biopsy, and HE staining, microscopic and histological examination were carried out. Results     Statistical results and biopsy showed that there was a good therapeutic effect on the rabbits′ acne models caused by propionibacterium acnes / oleic acid in the ALA-PDT treatment group (P<0.01 or P<0.05).The effect in the other two positive treatment groups was also obvious (P<0.05), and the therapeutic effect in the ALAPDT repeated treatment group was stronger than in the ALA-PDT single treatment group and the two positive drug groups. Conclusion      Repeated ALAPDT treatment has good therapeutic effect on rabbits′ acne models caused by  propionibacterium acnes/oleic acid.

Key words: Aminolevulinic acid hydrochloride; Acne; Animal, model; Photodynamic therapy; Effect;   Rabbits