醒脑静注射液对蛛网膜下腔出血后海马神经元凋亡的影响

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (7): 57-60.

醒脑静注射液对蛛网膜下腔出血后海马神经元凋亡的影响

郭晋辉1,姜勇2

山东大学 1．医学院，济南 250012； 2．附属济南市中心医院神经外科, 济南 250013

收稿日期:2010-12-10 出版日期:2011-07-10 发布日期:2011-07-10
通讯作者: 姜勇(1961- ),男,教授,主任医师,主要从事神经外科基础与临床研究。 E-mail: jyong9938@yahoo. com. cn
作者简介:郭晋辉(1977- ),男,硕士研究生,主要从事神经外科基础与临床研究。

Effect of Xingnaojing on apoptosis of hippocampal neurons in rats with subarachnoid hemorrhage

GUO Jin-hui1, JIANG Yong2

Shandong University 1. School of Medicine, Jinan 250012, China； 2. Department of Neurosurgery, Jinan Central Hospital Affiliated， Jinan 250013, China

Received:2010-12-10 Online:2011-07-10 Published:2011-07-10

摘要/Abstract

摘要：

目的探讨醒脑静注射液(XNJJ)对蛛网膜下腔出血(SAH)后海马相关因子凋亡的影响。方法选择成年雄性Wistar大鼠35只，随机分为空白对照组、生理盐水对照组、SAH组、XNJJ治疗1组、XNJJ治疗2组，每组7只，采用新鲜自体股动脉血枕大池二次注入法建立SAH模型，用Real-Time PCR技术检测海马神经元中凋亡分子Caspase-3和Bax以及抗凋亡分子Bcl-2在各组的变化情况，并用Western blot进一步验证此变化。结果 SAH后海马神经元中Caspase-3和Bax的表达均有不同程度上升(P＜0.05)，Bcl-2也有所上升(P＜0.01)。XNJJ治疗1组及2组Caspase-3和Bax的表达较SAH组下降(P＜0.01)，而Bcl-2变化无统计学差异(P＞0.05)。结论XNJJ可以抑制海马神经元凋亡，具有脑保护作用。

关键词: 蛛网膜下腔出血；模型，动物；海马；细胞凋亡；大鼠，Wistar

Abstract:

Objective To investigate the effect of Xingnaojing(xnjj) on the apoptosis of hippocampal neurons in rats with subarachnoid hemorrhage(SAH). Methods The SAH model was established by two time autologous blood injection into the cisterna magna of adult male Wistar rats. 35 rats were randomly divided into 5 groups naive, saline control, SAH, xnjj treatment 1, and xnjj treatment 2 groups. Gene expressions of Caspase-3, Bax, and Bcl-2 in different groups after SAH were detected with Real-Time PCR. Further, Western blot was applied to determine protein changes of these three molecules after SAH. Results Compared with the control group, Caspase-3, Bax, and Bcl-2 increased in varying degrees in the hippocampus of the SAH group(P＜0.05), while in Xingnaojing treatment groups, Caspase-3 and Bax, but not Bcl-2, decreased when compared with the SAH group(P＜0.01). Conclusion Xingnaojing shows an obvious neuroprotective effect on hippocampal neurons by inhibiting SAHrelated apoptosis.

Key words: Subarachnoid hemorrhage； Models, animal； Hippocampal; Apoptosis； Rats, Wistar

中图分类号:

R743.35

郭晋辉1,姜勇2. 醒脑静注射液对蛛网膜下腔出血后海马神经元凋亡的影响[J]. 山东大学学报(医学版), 2011, 49(7): 57-60.

GUO Jin-hui1, JIANG Yong2. Effect of Xingnaojing on apoptosis of hippocampal neurons in rats with subarachnoid hemorrhage[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(7): 57-60.

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