丙戊酸对前列腺癌PC3细胞自噬的影响

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (7): 44-47.

丙戊酸对前列腺癌PC3细胞自噬的影响

黄忠献，金讯波，王慕文，张沂南，郑懿，夏庆华

山东大学附属省立医院泌尿微创科，济南 250021

收稿日期:2010-01-18 出版日期:2011-07-10 发布日期:2011-07-10
通讯作者: 通讯作者：夏庆华(1971- )，男，主任医师，主要从事泌尿系肿瘤及多囊肾的临床及基础研究。 E-mail：xqhgege@hotmail.com
作者简介:黄忠献(1982- )，男，硕士研究生，主要从事泌尿系肿瘤学研究。
基金资助:
山东省科技厅立项项目(BS2O10YY047)。

Effect of valproic acid on autophagy in prostate cancer PC3 cells

HUANG Zhong-xian， JIN Xun-bo, WANG Mu-wen, ZHANG Yi-nan, ZHENG Yi, XIA Qing-hua

Minimally Invasive Urologic Center, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China

Received:2010-01-18 Online:2011-07-10 Published:2011-07-10

摘要/Abstract

摘要：

目的观察丙戊酸(VPA)诱导前列腺癌PC3细胞发生自噬性死亡的现象,初步探讨其发生的可能机制。方法 VPA作用前列腺癌PC3细胞后, 应用CCK-8试剂盒检测细胞生存率，透射电子显微镜及细胞免疫荧光测定仪观察细胞超微结构及自噬水平, Western-blotting检测PC3细胞内自噬特异性蛋白-Ⅱ(LC3-Ⅱ)及p-Akt蛋白的表达水平。结果经VPA处理后,前列腺癌PC3细胞活性短期内(≤4d)未受到明显抑制。透射电子显微镜观察可见，前列腺癌PC3细胞质内有大量自噬体和自噬溶酶体,其自噬水平随VPA作用时间的延长逐渐增强。Western-blotting检测表明，前列腺癌PC3细胞LC3-Ⅱ表达水平随VPA作用时间的延长明显升高,而p-Akt表达水平则明显降低。结论 VPA可诱导前列腺癌PC3细胞发生自噬,其诱导前列腺癌PC3细胞发生自噬的机制可能与阻断Akt/mTOR信号转导通路有关。

关键词: 丙戊酸；前列腺肿瘤；自噬

Abstract:

Objective To observe autophagy of prostate cancer PC3 cells induced by valproic acid(VPA)and explore the possible mechanism. Methods The prostate cancer PC3 cell line treated with VPA was used in this study. Cell survival rate was determined by cell counting kit8. Autophagosomes were observed under a transmission electronic microscope. The intensity of autophagy was analyzed by immunofluorescence. Expression levels of LC3-Ⅱ and p-Akt were measured by Western blot after VPA treatment. Results Viability of the prostate cancer PC3 cell line treated with acute VPA was not significantly inhibited in a short time(≤4d). Autophagosomes and autolysosomes were observed in the VPA-treated prostate cancer PC3 cell line under a transmission electronic microscope. Intensity of autophagy was in a time-dependent manner. Expression level of the related protein LC3-Ⅱwas elevated, while expression level of p-Akt declined following VPA treatment in the prostate cancer PC3 cell line. Conclusions VPA may induce autophagy in the prostate cancer PC3 cell line. The mechanism may be related to the disruption of the Akt/mTOR signaling pathway.

Key words: Valproic acid; Prostate tumor; Autophagy

中图分类号:

R697.32

黄忠献，金讯波，王慕文，张沂南，郑懿，夏庆华. 丙戊酸对前列腺癌PC3细胞自噬的影响[J]. 山东大学学报(医学版), 2011, 49(7): 44-47.

HUANG Zhong-xian， JIN Xun-bo, WANG Mu-wen, ZHANG Yi-nan, ZHENG Yi, XIA Qing-hua. Effect of valproic acid on autophagy in prostate cancer PC3 cells[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(7): 44-47.

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