关键词: 硫氢化钠；高浓度葡萄糖；胱硫醚-γ-裂解酶；原代人脐静脉内皮细胞

Abstract:

Objective     To observe the effect of sodium hydrosulfide on primary human umbilical vein endothelial cells (HUVECs)  cultured with high glucose. Methods     The 0.125% trypsin and 0.01% EDTA perfusion method was used to isolate HUVECs. Then, the cells were treated for another 72h with 5.5mmol/L glucose, 25mmol/L glucose, 25mmol/L glucose plus 50μmol/L NaHS or 50μmol/L NaHS. The MTT method was used for cells viability. The protein level of cystathionine gamma-lyase (CSE), a key synthase to produce H2S, was measured by Western blot. Results     MTT showed that compared to 5.5mmol/L glucose, cell viability of 25mmol/L glucose group decreased 28.37%(P<0.05). The 25mmol/L glucose plus 50μmol/L NaHS group rose 30.3% (P<0.05) compared to the 25mmol/L glucose group. There was no significant difference between the 50μmol/L NaHS  and the 5.5mmol/Lglucose group(P＞0.05). Western blot showed that 25mmol/L glucose could reduce protein expression of CSE(P＜0.05), compared to 5.5mmol/L glucose, and NaHS could ameliorate this phenomenon(P＜0.05). Conclusion      High glucose can lower cells viability and decrease expression of CSE in HUVECs and NaHS can ameliorate such effects.

Key words: Sodium hydrosulfide; High glucose; Cystathionine gamma-lyase; Primary human umbilical vein endothelial cells