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山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (5): 125-.

• 论文 • 上一篇    下一篇

肺结核患者血清SP-D检测的临床意义

徐勇,胡华,迟晶宇,杨燕,鞠云飞,侯宝青,李学政   

  1. 山东省胸科医院呼吸科, 济南 250013
  • 收稿日期:2011-02-18 出版日期:2011-05-10 发布日期:2011-05-10
  • 通讯作者: 胡华(1962- ),主任医师,主要从事呼吸系统相关疾病的研究。 E-mail:huhua2751@126.com
  • 作者简介:徐勇(1964- ),男,主管检验师,主要从事免疫等检验研究。 E-mail:Xuyong@xkyy.org
  • 基金资助:

    山东省科学技术厅科研基金资助项目(2006GG2202035)

Detection of SP-D in the serum from pulmonary tuberculosis patients

XU Yong, HU Hua, CHI Jing-yu, YANG Yan, JU Yun-fei, HOU Bao-qing, LI Xue-zheng   

  1. Department of Respiratory Diseases, Shandong Chest Hospital, Jinan 250013, China
  • Received:2011-02-18 Online:2011-05-10 Published:2011-05-10

摘要:

目的     检测肺结核患者血清中肺表面活性物质相关蛋白D (SPD)的表达,探讨其在肺结核诊断中的意义。方法     采用ELISA方法检测101例肺结核患者、30例慢性阻塞性肺疾病患者(COPD)、85例健康人血清中SPD的水平并测定血清结核抗体,沉降法检测血沉,电阻法测定白细胞计数、中性粒细胞比例、淋巴细胞比例,芒图试验法进行结核菌素试验(PPD试验),抗酸染色检测痰中结核菌数量。结果      肺结核患者血清中SPD的水平明显高于健康人、低于COPD患者(P<0.05);ROC曲线显示肺结核患者血清SPD的Cutoff值为0.26,在肺结核病诊断中敏感性为72.30%,特异性91.00%,诊断准确率为80.65%;PPD试验及结核抗体与血清SP-D具有相关性(χ2分别为8.44、17.08,P均<0.01)。结论    血清SP-D与肺结核疾病具有重要的相关性,有望成为肺结核诊断的一种辅助检测指标。

关键词: 结核,肺;血清学试验;肺表面活性物质相关蛋白D;诊断

Abstract:

Objective      To investigate the change of serum surfactant protein-D(SP-D) in pulmonary tuberculosis patients, observe its relation with gender, age and other factors, and explore value of diagnosis for pulmonary tuberculosis. MethodsThe level of SP-D in the serum was detected by ELISA in 101 cases of pulmonary tuberculosis, 30 cases of chronic obstructive pulmonary disease(COPD) and 85 healthy people. Leucocyte count, neutrophilic granulocyte percentage and lymphocyte percentage were detected with the electric resistance method, erythrocyte sedimentation rates by the sedimentation process, tubercle bacillus antibodies by ELISA, purified protein derivative(PPD) trial by the Mantoux method, and count of tubercle bacilli by acidfast stain. Results    The level of SP-D in the serum from pulmonary tuberculosis patients was significantly higher than that of the healthy,while it was significantly lower than that of COPD patients(P<0.05). The cutoff value of SP-D was 0.26 determined by the ROC curve drawn according to the measured values. The sensitivity, specificity and accuracy of detection of SP-D in the serum were 72.30%,  91.00%, and 80.65% respectively in the diagnosis of pulmonary tuberculosis. There were no obvious differences in SP-D levels in different conditions of gender, age, leukocyte count, neutrophilic granulocyte percentage, lymphocyte percentage, sedimentation process and count of tubercle bacilli in the sputum and hollow in the lung, however, there were  correlations of SP-D level with tubercle bacillus antibodies and PPD trial(values of  were 8.44 and 17.08 respectively,P<0.01). Conclusions     Detection of SP-D in serum is of great significance in the diagnosis of pulmonary tuberculosis, may become a new diagnostic method, and is worth exploring.

Key words: Tuberculosis, pulmonary; Serologic tests; Surfactant protein-D; Diagnosis

中图分类号: 

  • R521
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诊断结核性胸腔积液
[J]. 山东大学学报(医学版), 2012, 50(9): 79-82.
[2] 路超1,沈亚娟1,邓云峰2,王长印1,张炳昌1,范刚1,赵跃然3,刘义庆1,赵胜梅1,王子娥1,张春芝1,卢志明1 . 肺结核患者杀伤细胞免疫球蛋白样受体基因多态性分析[J]. 山东大学学报(医学版), 2012, 50(1): 122-.
[3] 胡华, 徐勇,迟晶宇, 鞠云飞, 杨燕,侯宝青,李学政,孟繁敏,李磊. 痰中SP-D水平的改变在肺结核诊断中的价值探讨[J]. 山东大学学报(医学版), 2010, 48(9): 79-81.
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