人参皂苷Rb1、Re对Aβ25-35诱导SK-N-SH细胞损伤的保护作用

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (4): 33-37.

人参皂苷Rb1、Re对Aβ_25-35诱导SK-N-SH细胞损伤的保护作用

贾立云1，潘晓华2，刘晶1，崔行3，王墨林1

1.山东大学医学院医学遗传学研究所，济南 250012； 2.山东省立医院普通外科，济南 250021；
3.山东大学医学院生物化学与分子生物学研究所，济南 250012

收稿日期:2011-01-24 出版日期:2011-04-10 发布日期:2011-04-10
通讯作者: 王墨林（1974- ），女，副教授，硕导，主要从事遗传病的基础研究。 E-mail：wml@sdu.edu.cn
作者简介:贾立云（1985- ），女，硕士研究生，主要从事神经退行性疾病的基础研究。 E-mail：jlyzk@sina.com
基金资助:
山东省科技发展计划资助项目（2009GG10002012）；山东省优秀中青年科学家科研奖励基金资助项目（2008BS03024）；国家自然科学基金青年科学基金资助项目（30500190）。

Protective effects of ginsenoside Rb1 and Re on SK-N-SH cells injured by Aβ_25-35

JIA Li-yun1, PAN Xiao-hua2, LIU Jing1, CUI Xing3, WANG Mo-lin1

1. Institute of Medical Genetics, School of Medicine, Shandong University, Jinan 250012, China;
2. Department of General Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China;
3. Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University, Jinan 250012, China

Received:2011-01-24 Online:2011-04-10 Published:2011-04-10

摘要/Abstract

摘要：

目的观察人参皂苷Rb1和Re对Aβ25-35诱导损伤SK-N-SH细胞的保护作用进而研究其内在机制。方法用Aβ25-35处理人神经母细胞瘤细胞(SK-N-SH细胞)，模拟阿尔茨海默病的神经元损伤，并以适当浓度人参皂苷Rb1或Re处理。采用MTT 法测定细胞存活率，荧光探针法检测细胞内ROS水平变化，Western blot 法检测tau蛋白磷酸化水平及活性GSK-3β蛋白表达。结果培养基中添加Aβ25-35后SK-N-SH细胞存活率明显下降，而人参皂苷Rb1和Re均能显著提高损伤细胞的存活率，降低细胞内ROS水平，降低活性GSK-3β的表达，造成tau蛋白396位点的磷酸化程度下降，缓解tau蛋白的过度磷酸化。结论人参皂苷Rb1和Re对Aβ25-35诱导损伤的SK-N-SH细胞具有一定的保护作用。

关键词: 人参属；阿尔茨海默病；tau蛋白类；Ca(2+)钙调蛋白依赖性蛋白激酶

Abstract:

Objective To investigate protective effects of ginsenoside Rb1 and Re against injury of SK-N-SH cells induced by Aβ_25-35 and the possible mechanism. Methods Aβ25-35 was added to the medium for cell culture to make a cell model of Alzheimer disease, and ginsenoside Rb1 or Re were used to treat the injured cells. Cell survival rates were determined by the MTT assay, intracellular ROS levels were determined by fluorescence probes, and expressions of phosphorylated tau and active glycogen synthase kinase 3β(GSK-3β) were determined by Western blot. Results The survival ratio of SK-N-SH cells were significantly decreased after exposure to Aβ_25-35, and treatment with ginsenoside Rb1 or Re significantly increased the survival ratio and decreased the cellular ROS level. Ginsenoside Rb1 and Re decreased expressions of phosphorylated tau and active GSK-3β, respectively. Conclusion Ginsenoside Rb1 and Re may exert a neuroprotective effect on SHNSH neural cells induced by neurotoxic Aβ_25-35.

Key words: Panax; Alzheimer disease; Tau proteins; Ca(2+)calmodulin dependent protein kinase

中图分类号:

R741

贾立云1，潘晓华2，刘晶1，崔行3，王墨林1. 人参皂苷Rb1、Re对Aβ_25-35诱导SK-N-SH细胞损伤的保护作用[J]. 山东大学学报(医学版), 2011, 49(4): 33-37.

JIA Li-yun1, PAN Xiao-hua2, LIU Jing1, CUI Xing3, WANG Mo-lin1. Protective effects of ginsenoside Rb1 and Re on SK-N-SH cells injured by Aβ_25-35[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(4): 33-37.

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