抗抑郁药物去甲替林在皮质神经元细胞氧糖剥夺模型中的保护作用及机制

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (4): 29-32.

抗抑郁药物去甲替林在皮质神经元细胞氧糖剥夺模型中的保护作用及机制

张文华，徐淑军，江玉泉，李刚，李新钢，周茂德，吴承远

山东大学齐鲁医院神经外科，山东大学脑科学研究所，济南 250012

收稿日期:2010-10-06 出版日期:2011-04-10 发布日期:2011-04-10
作者简介:张文华（1970- ），男，副教授，主要从事脑血管病和脑肿瘤治疗及发病机制研究。 E-mail：wzhang215@hotmail.com
基金资助:
国家自然科学基金资助项目（30872658）；山东省自然科学杰出青年基金资助项目（JQ200808）。

Protective effect of tricyclic antidepressant nortryptyline of cortical neurons on rats with OGD-induced injury and its mechanism

ZHANG Wen-hua, XU Shu-jun, JIANG Yu-quan, LI Gang, LI Xin-gang, ZHOU Mao-de, WU Cheng-yuan

Department of Neurosurgery, Qilu Hospital of Shandong University, Institute of Brain Science of Shandong University, Jinan 250012, China

Received:2010-10-06 Online:2011-04-10 Published:2011-04-10

摘要/Abstract

摘要：

目的探讨抗抑郁药物去甲替林对氧糖剥夺损伤皮质神经元的保护作用及其机制。方法采用体外培养的原代大鼠皮层神经元细胞，经不同浓度（1.0、2.5、5.0、10.0μmol/L）的去甲替林预处理后，建立氧糖剥夺模型。应用免疫细胞染色、MTT和台盼蓝染色检测去甲替林对神经元细胞状态、细胞活性、死亡率的影响。应用Western杂交测定去甲替林对线粒体细胞色素c、Smac/Diablo、AIF释放的影响。结果去甲替林能够明显改善氧糖剥夺损伤后神经元的生存状况，提高细胞存活率，并且明显抑制了损伤后神经元线粒体细胞色素c、Smac/Diablo、AIF的释放。结论抗抑郁药物去甲替林通过抑制细胞线粒体细胞凋亡因子（细胞色素c、Smac/Diablo、AIF）释放来保护氧糖剥夺损伤的皮质神经元，为抗抑郁药物去甲替林神经保护作用深入研究奠定了基础。

关键词: 去甲替林；神经元；氧糖剥夺；细胞凋亡诱导因子

Abstract:

Objective To investigate the protective effect and mechanism of nortryptyline on oxygen-glucose deprivation (OGD)-induced primary cortical neuronal death. Methods In vitro cultured primary cortical neurons were induced by OGD with nortryptyline in different concentrations（1.0,2.5,5.0,10.0μmol/L）, or without nortryptyline pretreatment. Then the neuronal activity and survival rate were determined by MTT， immunocytochemical staining and trypan blue staining. Also, release amounts of cytochrome c, Smac/Diablo and apoptosis-inducing factor (AIF) were determined by Western blot. Results Nortryptyline significantly improved the growth condition and survival rate of primary cortical neurons, and inhibited cytochrome c, Smac/Diablo and AIF release after OGD injury. Conclusion Nortryptyline plays a neuroprotective role in cortical neuronal death induced by OGD, via the inhibition of cytochrome c, Smac/Diablo and AIF release.

Key words: Nortryptyline; Neurons; Oxygen-glucose deprivation; Apoptosis

中图分类号:

R743.33

张文华，徐淑军，江玉泉，李刚，李新钢，周茂德，吴承远. 抗抑郁药物去甲替林在皮质神经元细胞氧糖剥夺模型中的保护作用及机制[J]. 山东大学学报(医学版), 2011, 49(4): 29-32.

ZHANG Wen-hua, XU Shu-jun, JIANG Yu-quan, LI Gang, LI Xin-gang, ZHOU Mao-de, WU Cheng-yuan. Protective effect of tricyclic antidepressant nortryptyline of cortical neurons on rats with OGD-induced injury and its mechanism[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(4): 29-32.

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