γ-氨基丁酸及其拮抗剂对γδT细胞的作用

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (4): 25-28.

γ-氨基丁酸及其拮抗剂对γδT细胞的作用

刘世育1，王营2，费素娟2，陈复兴3，刘军权3

1.徐州市第一人民医院消化内科, 江苏徐州 221002;
2.徐州医学院附属医院消化内科, 江苏徐州 221002;
3.中国人民解放军第97医院中心实验室, 江苏徐州 221004

收稿日期:2010-06-07 出版日期:2011-04-10 发布日期:2011-04-10
作者简介:刘世育(1982- ),男,硕士,住院医师,主要从事消化系统肿瘤基础与临床的研究。 E-mail：misslsy2005@yahoo.com.cn

Effects of γ-aminobutyric acid and its antagonist on γδT cells

LIU Shi-yu1, WANG Ying2, FEI Su-juan2, CHEN Fu-xing3, LIU Jun-quan3

1. Department of Gastroenterology, The First People′s Hospital of Xuzhou, Xuzhou 221002, Jiangsu, China;
2. Department of Gastroenterology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu, China；
3. Central Laboratory, The 97 th Hospital of Chinese PLA, Xuzhou 221004, Jiangsu, China

Received:2010-06-07 Online:2011-04-10 Published:2011-04-10

摘要/Abstract

摘要：

目的研究γ-氨基丁酸（γ-aminobutyric acid, GABA）及其A受体拮抗剂印防己毒素（picrotoxin，PTX）对人外周血γδT细胞的增殖、细胞表面受体的表达、杀伤活性的影响及其可能的作用机制。方法在体外用不同浓度的GABA和PTX与人γδT细胞作用后，用MTT比色法和流式细胞仪(FCM)以及乳酸脱氢酶(LDH)法分别检测人γδT细胞的增殖能力、T细胞表面受体的表达和杀伤活性的变化。结果 GABA能显著地抑制γδT细胞的生长(P＜0.01)，并具有浓度依赖性, PTX对GABA的抑制细胞增殖有拮抗作用；GABA显著降低γδT细胞表面受体NKG2D的表达, 增加γδ-TCR的表达, PTX有轻微的协同作用；GABA抑制了γδT细胞对人胰腺癌细胞株SW1990的杀伤活性, PTX能够拮抗GABA的此项作用。结论这些结果提示GABA可能通过多种途径对γδT细胞发挥作用，其杀伤活性主要由其表面受体NKG2D介导的, 并且需要γδT细胞同时表达γδTCR和NKG2D两种受体。

关键词: γ-氨基丁酸；印防己毒素；γδT细胞；杀伤活性

Abstract:

Objective To research effects of GABA and GABAA receptor antagonist (picrotoxin, PTX) on proliferation, surface receptor expression and cytotoxicity of human peripheral blood γδT cells, and possible mechanism of action. Methods After γδT cells were treated with different concentrations of GABA and PTX, the proliferation, surface receptor expression and cytotoxicity of γδT cells were detected by MTT, flow cytometry and the lactate dehydrogenase method, respectively. Results GABA could decrease proliferation of γδT cells(P＜0.01) in a dose-dependent manner. Expression of NKG2D on γδT cells was down-regulated by GABA, while TCR-γδ was up-regulated. GABA could inhibit γδT cells from killing the human pancreatic cancer cell line SW1990. PTX had an antagonistic role in the actions of GABA on the proliferation and cytotoxicity of γδT cells, but for surface receptor expression, PTX had a slightly synergistic effect. Conclusion GABA may affect γδT cells via multiple pathways, besides its a receptor. The cytotoxicity of γδT cells is mainly mediated by NKG2D, which may need expressions of both γδTCR and NKG2D.

Key words: γ-aminobutyric acid； Picrotoxin； γδT cells； Cytotoxicity

中图分类号:

R735.9

刘世育1，王营2，费素娟2，陈复兴3，刘军权3. γ-氨基丁酸及其拮抗剂对γδT细胞的作用[J]. 山东大学学报(医学版), 2011, 49(4): 25-28.

LIU Shi-yu1, WANG Ying2, FEI Su-juan2, CHEN Fu-xing3, LIU Jun-quan3. Effects of γ-aminobutyric acid and its antagonist on γδT cells[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(4): 25-28.

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