结直肠癌中p53、Nup88的表达及其相关性

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (4): 105-109.

结直肠癌中p53、Nup88的表达及其相关性

赵增仁1，张丽静1，张志勇2，郑峥1，王园园1，孙晓锋1

1.河北医科大学第一医院普外科，石家庄 050031； 2.唐山市工人医院病理科，河北唐山 063000

收稿日期:2010-09-03 出版日期:2011-04-10 发布日期:2011-04-10
作者简介:赵增仁（1969- ），男，博士，主任医师，主要从事消化道肿瘤的基础及临床研究。 E-mail: bmsc120@163.com
基金资助:
国家自然科学基金资助项目（30772115）。

Expressions and relationship of p53 and Nup88 in colorectal carcinoma

ZHAO Zeng-ren1, ZHANG Li-jing1, ZHANG Zhi-yong2, ZHENG Zheng1, WANG Yuan-yuan1, SUN Xiao-feng1

1. Department of General Surgery, First Hospital of Hebei Medical University, Shijiazhuang 050031, China;
2. Department of Pathology, Tangshan Workers′ Hospital, Tangshan 063000, Hebei, China

Received:2010-09-03 Online:2011-04-10 Published:2011-04-10

摘要/Abstract

摘要：

目的观察p53和Nup88 在结直肠癌（CRC）中的表达，探讨二者相互作用的关系。方法采用逆转录聚合酶链反应（RT-PCR）分别检测结肠癌细胞株（HCT-116p53+/+和HCT116p53-/-）中p53和Nup88 mRNA的表达，采用免疫组织化学法检测48例CRC及13例正常结直肠组织中p53和Nup88蛋白的表达。结果 HCT-116p53-/-细胞株中p53 mRNA的表达较HCT-116p53+/+明显下调，但Nup88 mRNA的表达在两者间无统计学差异；48例CRC中，p53和Nup88蛋白均呈高表达，阳性率分别为68.75%和72.92%，明显高于正常结直肠组织（P＜0.01）。但未发现二者存在相关性（r=0.173, P＞0.05）。结论 CRC中p53和Nup88蛋白均呈现高表达，二者均参与了结直肠癌的发生和发展，但未发现二者存在明显相关性。

关键词: 结直肠癌；p53；核孔蛋白88

Abstract:

Objective To study expressions of p53 and Nup88 and their correlation in patients with colorectal carcinoma(CRC). Methods Expressions of p53 and Nup88 mRNA were determined by reverse transcriptive-polymerase chain reaction (RTPCR) in HCT-116p53+/+ and HCT-116p53-/- cell lines. Protein expressions of p53 and Nup88 were detected in 48 CRC samples and 13 normal colon samples by immunohistochemistry. Results In the HCT-116p53-/- cell line, expression of p53 mRNA was more significantly decreased than that in HCT-116p53+/+, while there was no significant difference in expression of Nup88 mRNA between the two cell lines. Positive expressions of p53 (68.75%) and Nup88 (72.92%) proteins in CRC were notably higher than those in the normal tissues (P＜0.01). However, there was no correlation between p53 and Nup88 expressions (r=0.173, P＞0.05). Conclusion Over-expressions of p53 and Nup88 play a role in the development of CRC, while there is no significant correlation between them.

Key words: Colorectal carcinoma; p53; Nucleoporin 88

中图分类号:

R656.9

赵增仁1，张丽静1，张志勇2，郑峥1，王园园1，孙晓锋1. 结直肠癌中p53、Nup88的表达及其相关性[J]. 山东大学学报(医学版), 2011, 49(4): 105-109.

ZHAO Zeng-ren1, ZHANG Li-jing1, ZHANG Zhi-yong2, ZHENG Zheng1, WANG Yuan-yuan1, SUN Xiao-feng1. Expressions and relationship of p53 and Nup88 in colorectal carcinoma[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(4): 105-109.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed