灯盏乙素对MMT诱导SK-N-SH神经细胞凋亡的保护作用

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (3): 46-49.

灯盏乙素对MMT诱导SK-N-SH神经细胞凋亡的保护作用

秦文1，张剑1，刘爱香2，徐凌忠3

1.山东大学校医院，济南 250012； 2.山东枣庄市立医院，山东枣庄 277100；
3.山东大学公共卫生学院，济南 250012

收稿日期:2011-01-04 出版日期:2011-03-10 发布日期:2011-03-10
通讯作者: 徐凌忠（1964- ），教授，博士生导师，主要从事社会医学研究。
作者简介:秦文（1972- ），副主任医师，硕士研究生，主要从事社会医学及毒理学研究。
基金资助:
山东省科技攻关项目（2007GG20002003）。

Inhibitory effect of scutellarin on apoptosis induced by MMT in SK-N-SH neural cells

QIN Wen1, ZHANG Jian1, LIU Ai-xiang2, XU Ling-zhong3

1. School Hospital, Shandong University, Jinan 250012, China; 2. Zaozhuang Municipal Hospital,
Zaozhuang 277100, Shandong, China; 3.School of Public Health, Shandong University, Jinan 250012, China

Received:2011-01-04 Online:2011-03-10 Published:2011-03-10

摘要/Abstract

摘要：

目的研究灯盏乙素对新型大气污染物甲基环戊二烯羰基锰（MMT）诱导人SK-N-SH细胞凋亡的保护作用及可能机制。方法将SK-N-SH细胞随机分为5组：正常组，MMT诱导组，1.2×10-1mg/L灯盏乙素组，3.0×10-2mg/L灯盏乙素组，7.5×10-3mg/L灯盏乙素组。灯盏乙素预保护2h，1mmol/L MMT诱导24h后，MTT法检测细胞存活率，分光光度法检测细胞活性氧（ROS）生成、细胞培养液和细胞内超氧化物歧化酶（SOD）活性及丙二醛（MDA）产量；化学发光法检测细胞三磷酸腺苷（ATP）释放量及Caspase3活性变化。结果灯盏乙素预保护的细胞在MMT诱导后，与MMT诱导组相比，细胞存活率上升（P＜0.05），细胞ROS生成量受到抑制（P＜0.01）；细胞培养液和细胞内SOD活性增高（P＜0.05），MDA产量降低（P＜0.01）；在这一过程中，细胞ATP的释放量有所恢复（P＜0.05），同时Capase-3 活性受到抑制（P＜0.05）。结论灯盏乙素通过抗氧化作用，抑制MMT对SK-N-SH细胞的凋亡作用。

关键词: 灯盏乙素；甲基环戊二烯羰基锰；神经细胞；凋亡

Abstract:

Objective To explore the inhibitory effect of scutelalrin on methylcyclopentadienyl manganese tricarbonyl (MMT)-induced apoptosis in SK-N-SH cells and its possible mechanism. Methods SK-N-SH cells were randomly grouped into 5 groups: the normal group, the MMT induction group, the 1.2×10-1mg/L scutellarin group， the 3.0×10-2mg/L scutellarin group and the 7.5×10-3mg/L scutellarin group. After incubation with scutelalrin for 2h， SKNSH cells were induced by 1mmol/L of MMT for 24h. Then viability of cells was determined the MTT assay. The production of reactive oxygen species (ROS) and malondialdehyde (MDA) and activity of superoxide dismutase (SOD) were determined by a spectrophotometer. The release of adenosine triphosphate(ATP) and activity of Caspase-3 were measured by chemiluminescence. Results After incubation with scutelalrin, the viability of cells markedly ascended（P＜0.05）. The production of ROS and MDA was inhibited （P＜0.01）, while the activity of SOD was increased（P＜0.05）. The release of ATP partly resumed（P＜0.05）, while the activity of Caspase-3 was significantly inhibited（P＜0.01）. Conclusion Scutelalrin can inhibit apoptosis of SK-N-SH cells induced by MMT through anti-oxidation in this process.

Key words: Scutelalrin; Methylcyclopentadienyl manganese tricarbonyl; Neuron; Apoptosis

中图分类号:

R963

秦文1，张剑1，刘爱香2，徐凌忠3. 灯盏乙素对MMT诱导SK-N-SH神经细胞凋亡的保护作用[J]. 山东大学学报(医学版), 2011, 49(3): 46-49.

QIN Wen1, ZHANG Jian1, LIU Ai-xiang2, XU Ling-zhong3. Inhibitory effect of scutellarin on apoptosis induced by MMT in SK-N-SH neural cells[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(3): 46-49.

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