Aβ1-42对胆碱能神经元KATP通道离子流影响的研究

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (3): 18-.

Aβ1-42对胆碱能神经元KATP通道离子流影响的研究

谭淑慧1，任晓燕1，李景新2，姚伟2，马国诏1

山东大学 1.附属省立医院神经内科，济南 250021; 2.医学院生理学研究所，济南 250012

收稿日期:2010-11-02 出版日期:2011-03-10 发布日期:2011-03-10
通讯作者: 马国诏（1973- ），男，博士，主要从事老年性痴呆、帕金森病和脑血管疾病发病机制与防治的研究。E-mail：maguozhao@hotmail.com
作者简介:谭淑慧（1984- ）,女，硕士研究生,主要从事老年性痴呆发病机制与防治的研究。 E-mail: xiaotan521.lovely@163.com
基金资助:
国家自然科学基金资助项目（No. 30870874）。

Effects of Aβ1-42 on the current of KATP channels in cultured cholinergic neurons

TAN Shu-hui1, REN Xiao-yan1, LI Jing-xin2, YAO Wei2, MA Guo-zhao1

1. Department of Neurology, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China;
2. Department of Physiology, School of Medicine, Shandong University, Jinan 250012, China

Received:2010-11-02 Online:2011-03-10 Published:2011-03-10

摘要/Abstract

摘要：

目的研究β淀粉样蛋白（Aβ1-42）对胆碱能神经元ATP敏感钾离子（KATP）通道离子流的影响。方法原代培养出生24h内的乳鼠皮层和海马胆碱能神经元，采用膜片钳全细胞记录技术记录Aβ1-42对单个胆碱能神经元KATP通道离子流的影响。结果与对照组比较，给予Aβ1-42后胆碱能神经元外向电流显著减少（P＜0.05），再给予ATP敏感性钾通道开放剂二氮嗪后此减小的外向电流无显著变化（P＞0.05）。结论 Aβ1-42对神经元KATP通道具有抑制作用。

关键词: 淀粉样β蛋白；钾通道；膜片钳术；大鼠，Wistar

Abstract:

Objective To study the effect of β-amyloid peptides (Aβ1-42) on the current of ATP-sensitive potassium(KATP) channels in cultured cortical and hippocampal cholinergic neurons in rats. Methods Cortical and hippocampal cholinergic neurons from neonatal rats less than 24 h after birth were primarily cultured. Membrane currents were measured using patch clamp whole cell recordings. Results Compared with the controls, the outward current of neurons significantly decreased after using Aβ1-42（P＜0.05）. and there was no obvious change after giving diazoxide, an opener of KATP channels（P＞0.05）. Conclusion Aβ1-42 inhibits the opening of KATP channels.

Key words: Amyloid beta-protein； Potassium channels; Patch-clamp techniques; Rats, Wistar

中图分类号:

R741.02

谭淑慧1，任晓燕1，李景新2，姚伟2，马国诏1. Aβ1-42对胆碱能神经元KATP通道离子流影响的研究[J]. 山东大学学报(医学版), 2011, 49(3): 18-.

TAN Shu-hui1, REN Xiao-yan1, LI Jing-xin2, YAO Wei2, MA Guo-zhao1. Effects of Aβ1-42 on the current of KATP channels in cultured cholinergic neurons[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(3): 18-.

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