束缚-浸水应激对大鼠迷走背核胆碱能神经元活动的影响

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (3): 13-17.

束缚-浸水应激对大鼠迷走背核胆碱能神经元活动的影响

赵东芹，艾洪滨

山东师范大学生命科学学院生理学教研室，济南 250014

收稿日期:2010-12-06 出版日期:2011-03-10 发布日期:2011-03-10
通讯作者: 艾洪滨（1951- ），男，教授，博导，主要从事神经生理学的研究。 E-mail: aihongbin@sdnu.edu.cn
作者简介:赵东芹（1978- ），女，讲师，硕士，主要从事环境动物学和动物生理学的研究。 E-mail: zhaodongqin78@163.com
基金资助:
国家自然科学基金资助项目(30770277、 30970354、31071920)。

Influence of restraint water-immersion stress on activity of cholinergic neurons in the dorsal motor nucleus of the vagus in the rat

ZHAO Dong-qin, AI Hong-bin

Department of Physiology, School of Life Science, Shandong Normal University, Jinan 250014, China

Received:2010-12-06 Online:2011-03-10 Published:2011-03-10

摘要/Abstract

摘要：

目的研究束缚-浸水应激（RWIS）大鼠迷走神经背核（DMV）中胆碱能神经元的活动情况。方法随机将10只雄性Wistar大鼠分为对照组和应激组，采用Fos和胆碱乙酰化酶（ChAT）免疫组织化学双标技术，统计对照组和应激组Fos、ChAT、Fos/ChAT双标阳性神经元数目。结果与对照组相比，应激组大鼠DMV大量神经元表达Fos，Fos阳性神经元主要集中于DMV尾段和吻段（P<0.01）；ChAT阳性神经元主要分布于DMV中段和尾段，应激组大鼠DMV中单位面积内ChAT阳性神经元数目减少（P<0.01）；Fos/ChAT双标阳性神经元的分布与Fos阳性神经元分布情况相似，RWIS组Fos/ChAT双标阳性神经元数目显著增加（P<0.01），Fos/ChAT双标阳性神经元占ChAT阳性神经元的比例在对照组和RWIS 组中分别为7.17%、21.12%（P<0.01）。结论 DMV中胆碱能神经元参与RWIS调控过程。

关键词: 应激；迷走神经背核；胆碱0-乙酰转移酶;Fos；大鼠

Abstract:

Objective To investigate activity of cholinergic neurons in the dorsal motor nucleus of the vagus (DMV) in response to water-immersion stress (RWIS) in rats. Methods Ten rats were randomly divided into the control group and the RWIS group. Fos-immunoreactive (Fos-IR), choline acetyltransferase immunoreactive (ChATIR) and double labeled (Fos/ ChAT-IR) neurons were counted using dual Fos and ChAT immunohistochemistry. Results Compared with unstressed rats, FosIR neurons dramatically increased in the DMV of RWIS rats (P<0.01), and Fos expression was higher in the caudal portion of the NTS compared with the rostral and intermedial portions. ChAT-IR neurons decreased and were mainly observed in the caudal and intermediate portions of the DMV in RWIS rats（P<0.01）. Percentages of Fos/ ChAT-IR norons in ChAT-IR neurons in unstressed and RWIS rats were 7.17% and 21.12%, respectively（P<0.01）. Conclusion Cholinergic neurons in DMV are involved in the stress response.

Key words: Stress; Dorsal nucleus of vagus; Choline 0-acetyltransferase; Fos; Rats

中图分类号:

赵东芹，艾洪滨. 束缚-浸水应激对大鼠迷走背核胆碱能神经元活动的影响[J]. 山东大学学报(医学版), 2011, 49(3): 13-17.

ZHAO Dong-qin, AI Hong-bin . Influence of restraint water-immersion stress on activity of cholinergic neurons in the dorsal motor nucleus of the vagus in the rat[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(3): 13-17.

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